DescriptionAll SUMO isoforms are translated with additional C-terminal residues that have to be removed to generate the active protein. Pro-SUMO-2 (95 amino acids) is the inactive precursor of SUMO-2 (93amino acids) and is processed at the C-terminus by SUMO-2 specific proteases (SENPs). The resulting SUMO-2 protein has the conserved C-terminal Gly-Gly residues that function in activation and conjugation reactions. This protein can be used as a negative control in sumoylation reactions or as a substrate for SENPs.Molecular Weight (kDa)10.9 kDaFormulation50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT, 10% glycerolSourceExpressed in E.coli expression systemSize100 µgStorage Temp & ConditionsStore at -80°C. Avoid multiple freeze/ thaw cycles.Purification NotesPurity: >95% by SDS-PAGEApplication NotesTypical in vitro concentrations are 10-50 μM depending on conditions