| ReagentType: | Other |
| 100%Guarantee: | 6months |
Description
DTT,alsoknownasCleland"sReagent,isaparticularlystrongreducingagent,owingtoitshighconformationalproprensitytoformasixmemberringwithaninternaldisulfidebond. Ithasaredoxpotentialof-0.33V@pH7.Itisused formanyproteinresearchapplications,includingreducingpolyacrylamideelectrophoresis(SDS-PAGE).The500mMstocksolution canbedirectlyusedtoachievetheamountofreducingagentrequiredforyourspecificapplication.
MolecularWeight(kDa)
154kDa
Formulation
10mMphosphatebufferedsaline,pH7.4
Concentration/Volume
500mM,1ml
StorageTemp&Conditions
>6monthsat4°C.
ApplicationNotes
Usedinbiochemistryandvarioussamplepreparationapplications,workstoreducedissulfidebridges,protectsbiomolecules,anddenaturesproteinsbeforeelectrophoresisanalysis(SDS-PAGE).1-10mMtomaintainreducedproteinsinsolution,50-100mMforcompletereductionforelectrophoresis.
