Description
DTT,alsoknownasCleland"sReagent,isaparticularlystrongreducingagent,owingtoitshighconformationalproprensitytoformasixmemberringwithaninternaldisulfidebond. Ithasaredoxpotentialof-0.33V@pH7.Itisused formanyproteinresearchapplications,includingreducingpolyacrylamideelectrophoresis(SDS-PAGE).The500mMstocksolution canbedirectlyusedtoachievetheamountofreducingagentrequiredforyourspecificapplication.

MolecularWeight(kDa)
154kDa

Formulation
10mMphosphatebufferedsaline,pH7.4

Concentration/Volume
500mM,1ml

StorageTemp&Conditions
>6monthsat4°C. 

ApplicationNotes
Usedinbiochemistryandvarioussamplepreparationapplications,workstoreducedissulfidebridges,protectsbiomolecules,anddenaturesproteinsbeforeelectrophoresisanalysis(SDS-PAGE).1-10mMtomaintainreducedproteinsinsolution,50-100mMforcompletereductionforelectrophoresis.