>
产品中心 >
acid_base_buffer_solution >
Epigentek/Methyamp热Taq EvaGreen qPCR混合物（无ROX）/R12028-1/1 ml
| ReagentType: | PCRMix |
| 100%Guarantee: | 6months |
Description
MethylampHotTaqEvaGreen®qPCRMix(noROX)isoptimizedforreal-timequantitativePCRassays.Theready-to-usemixincludesMethylampHotTaqDNApolymerase,ultrapuredNTPs,MgCl2andEvaGreen®dye.Onlywater,templateandprimersneedtobeadded.MethylampHotTaqDNApolymeraseisactivatedbya15minincubationstepat95°C.Thispreventsextensionofnonspecificallyannealedprimersandprimer-dimersformedatlowtemperaturesduringqPCRsetup.
EvaGreen®Dye:DNA-bindingdyeforqPCRthat,comparedtoSYBR®GreenI,hassimilarspectrabutmuchlessPCRinhibition.Itisextremelystabledyeandhasbeenshowntobenonmutagenicandnoncytotoxic.EvaGreen®iscompatIBLewithallcommonreal-timePCRcyclers–simplyselectthestandardsettingsforSYBR®GreenorFAM!
MethylampHotTaqEvaGreen®qPCRMix(noROX)canbeusedwithallmajorqPCRcyclersthatdonotrequirereferencedye,includingBio-RadiQ™5,Opticon™,Opticon™2;Chromo4™,MiniOpticon,CFX96,CFX384;CorbettRotor-Gene™3000,Rotor-Gene™6000;RocheLightCycler®480andTechneQuantica™.
Composition
- MethylampHotTaqDNApolymerase
- 5xqPCRBufferE
- 12.5mMMgCl2:1xPCRsolution–2.5mMMgCl2
- dNTPs,includingdTTPtoimprovereactionsensitivityandefficiencycomparedtodUTP
- EvaGreen®dye
- noROXdye
- 1mlofmixissufficientfor250reactions
Applications
- DetectionandquantificationofDNAandCDNAtargetsincludingDNAfrombisulfitetreatment,ChIPorMeDIP
- Profilinggeneexpression
- HighResolutionMelt(HRM)Analysis
- Microbialdetection
- Viralloaddetermination
StorageConditions
Routinestorage:-20°C.Shippingandtemporarystorageforupto1monthatroomtemperaturehasnodetrimentaleffectsonthequalityofMethylampHotTaqEvaGreen®qPCRMix(noROX).
