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Epigentek/Methyamp热Taq EvaGreen qPCR混合物（ROX）/R12027-1/1 ml
| ReagentType: | PCRMix |
| 100%Guarantee: | 6months |
Description
MethylampHotTaqEvaGreen®qPCRMix(ROX)isoptimizedforreal-timequantitativePCRassays.Theready-to-loadmixincludesMethylampHotTaqDNApolymerase,ultrapuredNTPs,MgCl2,EvaGreen®dyeandROXdyeaccordingtosystemrequirements.Onlywater,templateandprimersneedtobeadded.MethylampHotTaqDNApolymeraseisactivatedbya15minincubationstepat95°C.Thispreventsextensionofnonspecificallyannealedprimersandprimer-dimersformedatlowtemperaturesduringqPCRsetup.
EvaGreen®Dye:DNA-bindingdyeforqPCRthat,comparedtoSYBR®GreenI,hassimilarspectrabutmuchlessPCRinhibition.Itisextremelystableandhasbeenshowntobenonmutagenicandnoncytotoxic.EvaGreen®iscompatIBLewithallcommonreal-timePCRcyclers–simplyselectthestandardsettingsforSYBR®GreenorFAM!
ROXDye:ROXisaninternalpassivereferencedyeusedtonormalizethefluorescentreportersignalgeneratedinqPCR.
Composition
- MethylampHotTaqDNApolymerase
- 5xqPCRBufferE
- 12.5mMMgCl2:1xPCRsolution–2.5mMMgCl2
- dNTPs,includingdTTPtoimprovereactionsensitivityandefficiencycomparedtodUTP
- EvaGreen®dye
- ROXdye
- 1mlofmixissufficientfor250reactions
Applications
- DetectionandquantificationofDNAandCDNAtargetsincludingDNAfrombisulfitetreatment,ChIPorMeDIP
- Profilinggeneexpression
- HighResolutionMelt(HRM)Analysis
- Microbialdetection
- Viralloaddetermination
StorageConditions
Routinestorage:-20°C.Shippingandtemporarystorageforupto1monthatroomtemperaturehasnodetrimentaleffectsonthequalityofMethylampHotTaqEvaGreen®qPCRMix(ROX).
