| ReagentType: | PCRMix |
| 100%Guarantee: | 6months |
Description
MethylampHotTaqProbeqPCRMix(noROX)isoptimizedforreal-timequantitativePCRassaysandcontainsallthecomponentsnecessarytoperformqPCR,withtheexceptionoftemplate,primers,andprobe.TheqPCRMixcontainsoptimizedcomponentsandMethylampHotTaqDNApolymerasesuppliedinaproprietaryreactionbufferthatenablesdetectionoflowcopynumbertargets.MethylampHotTaqDNApolymeraseisactivatedbya15minincubationstepat95°C.Thispreventsextensionofnon-specificallyannealedprimersandprimer-dimersformedatlowtemperaturesduringqPCRsetup.
MethylampHotTaqProbeqPCRMix(noROX)iscompatIBLeforusewithanyprobesystemandqPCRcyclersthatdonotrequirereferencedye,includingBio-RadiQ™5,Opticon™,Opticon™2;Chromo4™,MiniOpticon,CFX96,CFX384;CepheidSmartCycler™;CorbettRotor-Gene™3000,Rotor-Gene™6000;EppendorfMastercycler®eprealplex;RocheLightCycler®480andTechneQuantica™.
Composition
- MethylampHotTaqDNApolymerase
- 5xqPCRbufferP
- 15mMMgCl2:1xPCRsolution–3mMMgCl2
- dNTPs,includingdTTPtoimprovereactionsensitivityandefficiencycomparedtodUTP
- NoROXdye
- 1mlofmixissufficientfor250reactions
Applications
- DetectionandquantificationofDNAandCDNAtargetsincludingDNAfrombisulfitetreatment,ChIPorMeDIP
- Profilinggeneexpression
- HighResolutionMelt(HRM)Analysis
- Microbialdetection
- Viralloaddetermination
StorageConditions
Routinestorage:-20°C.Shippingandtemporarystorageforupto1monthatroomtemperaturehasnodetrimentaleffectsonthequalityofMethylampHotTaqProbeqPCRMix.
