Description
MethylampHotTaqProbeqPCRMixisoptimizedforreal-timequantitativePCRassaysandcontainsallthecomponentsnecessarytoperformqPCR,withtheexceptionoftemplate,primers,andprobe.TheqPCRMixcontainsoptimizedcomponentsandMethylampHotTaqDNApolymerasesuppliedinaproprietaryreactionbufferthatenablesdetectionoflowcopynumbertargets.MethylampHotTaqDNApolymeraseisactivatedbya15minincubationstepat95°C.Thispreventsextensionofnon-specificallyannealedprimersandprimer-dimersformedatlowtemperaturesduringqPCRsetup.

MethylampHotTaqProbeqPCRMix(ROX)iscompatIBLeforusewithanyprobesystemandqPCRcyclersrequiringhighROXdyelevel,includingABIPRISM®5700,7000,7300,7700,7900and7900HT(includingFast-Block);StratageneMx3000P™,Mx3005P™andMx4000®.

Composition

• MethylampHotTaqDNApolymerase 
• 5xqPCRbufferP 
• 15mMMgCl2:1xPCRsolution–3mMMgCl2 
• dNTPs,includingdTTPtoimprovereactionsensitivityandefficiencycomparedtodUTP 
• ROXdye(ROXdyeisaninternalpassivereferencedyeusedtonormalizethefluorescentreportersignalgeneratedinqPCR.)
• 1mlofmixissufficientfor250reactions

Applications

• DetectionandquantificationofDNAandCDNAtargetsincludingDNAfrombisulfitetreatment,ChIPorMeDIP
• Profilinggeneexpression 
• HighResolutionMelt(HRM)Analysis
• Microbialdetection
• Viralloaddetermination

StorageConditions
Routinestorage:-20°C.Shippingandtemporarystorageforupto1monthatroomtemperaturehasnodetrimentaleffectsonthequalityofMethylampHotTaqProbeqPCRMix.