Description
EpiQuikRedTaq2xPCRMixisapremixedsolutioncontainingeverythingneededforsuccessfulPCRreactionexceptspecificprimersandDNAtemplate.Themixincludeshigh-qualityrecombinantEpiQuikRedTaqHotThermostableDNApolymerase,nucleotidesandmagnesiuminaPCRreactionbuffer.Themixhasaspecialadditive-theinertreddye.Thismakesitverysuitableforstandardapplications.Strongredcolorofthemixallowsusertoverifyadequatemixing.ReactionproductsarereadyfordirectgelloADIngandthedyeservesasaMarkerforelectrophoresisprogressmonitoring.Forthereactionset-up,addthePCRMix(10or25µl)totheprimers,templateandwaterforthetotalreactionvolumeof20or50µl.ToactivatetheEpiQuikRedTaqHotThermostableDNApolymeraseitshouldbeincubatedat95-97°Cfor15minutesasafirstPCRstep.ThisenzymemixallowsthePCRsetupatambienttemperaturewithoutnonspecificannealingandextension.

Composition

• EpiQuikRedTaqDNAPolymerase(0.1U/µl)inreactionbuffer,4mMMgCl2,0.4mMofeachdNTPs(dATP,dCTP,dGTP,dTTP)
• Water(nucleasefree)-1ml
• 1mlofmixissufficientfor100reactions

Applications

• Ready-to-usemixreducesexperimentalvariABIlity
• Reducedpipettingsteps,therebylesstime-consumingset-upofPCR
• Set-upatroomtemperature
• Visualizationofcompletereactionmixing
• Directloadingofsamplesfollowingamplification

StorageConditions
Storeat-20ºC.Guaranteedstablefor1yearwhenproperlystored.