
Epigentek/Methylamp H减去M-MLV逆转录酶/R12017-1/10000单位
商品编号:
R12017-1
市场价:
¥5740.00
美元价:
3444.00
产品分类:
酸碱缓冲液
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
ReagentType: | PCREnzyme |
100%Guarantee: | 6months |
ProductOverview
Description
MethylampHMinusM-MLVReverseTranscriptaseisageneticallymodifiedM-MLVRTwhichexhibitsRNAorDNAdependentDNApolymerase,butlacksribonucleaseHactivity.ThisenzymecansynthesizeacomplementaryDNAstrandinitiatingfromaprimerusingRNAorDNAtemplates.RemovaloftheRNaseHactivityresultsinanincreaseoffull-lengthCDNAproducts.TheenzymehasRNApolymerization-dependentandDNApolymerization-dependentactivitybutlacksribonucleaseHactivity.
Concentration
200units/µl(oneunitisdefinedastheamountofenzymerequiredtocatalyzetheincorporationof 1nmolofdTTPintoanacid-insolubleformin10minutesat37°C).
Composition
- MethylampHMinusM-MLVReverseTranscriptase
- 5xRTReactionBuffer1(withMgCl2andDTT):0.25MTris-HCl,0.5MKCl,30mMMgCl2,25mMDTT
- 5xRTReactionBuffer2(Mg2+andDTTfree):0.25MTris-HCl,0.5MKCl
- 25mMMgCl2
- 20mMMnCl2
- 100mMDTT
Applications
- cDNAsynthesis
- RNAanalysisbyprimerextension
- DNAlabeling
StorageConditions
50%glycerol(v/v),50mMTris-HClpH8.0at25ºC,100mMNaCl,5mMDTT,1mMEDTA,0.1%NP-40.Storeat-20°C.
品牌介绍
Epigentek快速,有效地制备DNA,蛋白质和染色质对于进行下游分析至关重要。我们的样品制备套件套件几乎适用于所有工作流程,无论是为ChIP-Seq或ChIP-PCR应用分离染色质,还是从各种起始原料制备亚硫酸氢盐-Seq或MS-PCR的DNA。我们的样品制备套件使您可以直接从提取到反应,为您的实验工作流程提供无数种可能性和灵活性。我们的DNA,蛋白质和染色质制备试剂盒是无数研究机会和下游应用的起点。