Description
MethylampThermoDNAPolymeraseisahighlyprocessivethermostableDNApolymerasesuitedforMALDI-TOFmassspectrometryandotherprimerextensionplatforms.Theenzymehas5’→3’polymerization-dependentexonucleasereplacementactivityONLYandenhancedefficiencyforincorporatingunconventionalnucleotides(labeledddNTPs&acyclonucleotides).

Concentration
5units/µl(oneunitisdefinedastheamountofenzymerequiredtocatalyzetheincorporationof10nmolofdNTPintoanacid-insolubleformin30minat74°C).

Source
PurifiedfromanE.colistraincarryinganoverproducingplasmidcontainingamodifiedgeneofThermusaquaticusDNAPolymerase.

Storage&DilutionBuffer
50%glycerol(v/v),20mMTris-HClpH8.7at25°C,100mMKCl,0.1mMEDTAandstABIlizers.

Composition

• MethylampThermoDNAPolymerase
• 10xReactionBufferThermo:500mMTris-HClpH9.5at25ºC 
• 100mMMgCl2

Applications

• Primerextension
• MALDI-TOFmassspectrometry
• Mass-array

StorageConditions
Temporarystorageforupto1monthatroomtemperaturehasnodetrimentaleffectsonthequalityofthisreagent.However,routinestorageat-20°Cisstronglyrecommended.