Description
MethylampTaqisahighlyprocessive,ThermostableDNApolymerase.DuetoitsgeneticmodificationsMethylampTaqhasanenhancedstABIlityatroomtemperaturewithnoactivitylossforupto1month.Theenzymehas5’→3’polymerization-dependentexonucleasereplacementactivitybutlacks3’→5’exonucleaseactivity.

Concentration
5units/µl(oneunitisdefinedastheamountofenzymerequiredtocatalyzetheincorporationof10nmolofdNTPintoanacid-insolubleformin30minat74°C)

Source
PurifiedfromanE.colistraincarryinganoverproducingplasmidcontainingamodifiedgeneofThermusaquaticusDNAPolymerase.

Storage&DilutionBuffer
50%glycerol(v/v),20mMTris-HClpH8.7at25°C,100mMKCl,0.1mMEDTAandstabilizers.

Composition

• MethylampTaqDNAPolymerase 
• 10xReactionBuffer1(Mg2+free):800mMTrisHCl,200mM(NH4)2SO4,0.2%w/vTween-20 
• 25mMMgCl2

Applications

• SuitedforawiderangeofPCRassays 
• Primerextension
• TAcloning

StorageConditions
Temporarystorageforupto1monthatroomtemperaturehasnodetrimentaleffectsonthequalityofthisreagent.However,routinestorageat-20°Cisstronglyrecommended.

Fig.1. Amplificationof420bptargetDNAusingMethylampTaqDNAPolymerase.

M–EpiQuik100bpDNALadder

1-3–MethylampTaqDNAPolymerase