Description
EpiQuikPfuDNAPolymerasehasbeenpurifiedfromtheRecombinantE.colistrainwithclonedgeneencodingPyrococcusfuriosusDNApolymerase.Inadditionto5´→3´DNApolymeraseactivity,EpiQuikPfuDNAPolymerasealsopossesses3´→5´exonuclease(proof-reADIng)activity.EpiQuikPfuDNAPolymeraseexhibitsthelowesterrorrateofanyThermostableDNApolymerasestudied,itisuptotenfoldmoreaccuratethannormalTaqDNApolymerase.Consequently,EpiQuikPfuDNAPolymeraseisusefulforpolymerizationreactionsrequiringhigh-fidelitysynthesis.

Concentration
5units/µl(oneunitoftheenzymecatalyzestheincorporationof10nanomolesofdeoxyribonucleotidesintoapolynucleotidefractionin30minat70°C).

Source
RecombinantE.colistrainwithclonedgeneencodingPyrococcusfuriosusDNApolymerase.

Composition

• EpiQuikPfuDNAPolymeraseinStorageBuffer:20mMTris-HCl(pH8.0),1mMDTT,0.1mMEDTA,100mMKCl,0.5%NonidetP40,0.5%Tween20and50%glycerol 
• 10xPfuBuffer:200mMTris-HCl(pH8.8at25°C),100mMKCl,100mM(NH4)2SO4,1%TritonX-100,1mg/mlBSA 
• 10xPfuBufferwithMgSO4:200mMTris-HCl(pH8.8at25°C),100mMKCl,100mM(NH4)2SO4,1%TritonX-100,1mg/mlBSA,20mMMgSO4 
• 25mMMgSO4Solution

Applications

• ClosetotentimesmoreaccuratethannormalDNApolymerase 
• Producesblunt-endedamplificationproductstobeusedforcloning 
• Remainsactiveevenafterincubating90minutesat95°C

StorageConditions
Storeat-20°C.Guaranteedstablefor12monthswhenproperlystored.