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Epigentek/EpiQuik Pfu DNA Polymerase/R12013-2/1000 Units
| ReagentType: | PCREnzyme |
| 100%Guarantee: | 6months |
Description
EpiQuikPfuDNAPolymerasehasbeenpurifiedfromtheRecombinantE.colistrainwithclonedgeneencodingPyrococcusfuriosusDNApolymerase.Inadditionto5´→3´DNApolymeraseactivity,EpiQuikPfuDNAPolymerasealsopossesses3´→5´exonuclease(proof-reADIng)activity.EpiQuikPfuDNAPolymeraseexhibitsthelowesterrorrateofanyThermostableDNApolymerasestudied,itisuptotenfoldmoreaccuratethannormalTaqDNApolymerase.Consequently,EpiQuikPfuDNAPolymeraseisusefulforpolymerizationreactionsrequiringhigh-fidelitysynthesis.
Concentration
5units/µl(oneunitoftheenzymecatalyzestheincorporationof10nanomolesofdeoxyribonucleotidesintoapolynucleotidefractionin30minat70°C).
Source
RecombinantE.colistrainwithclonedgeneencodingPyrococcusfuriosusDNApolymerase.
Composition
- EpiQuikPfuDNAPolymeraseinStorageBuffer:20mMTris-HCl(pH8.0),1mMDTT,0.1mMEDTA,100mMKCl,0.5%NonidetP40,0.5%Tween20and50%glycerol
- 10xPfuBuffer:200mMTris-HCl(pH8.8at25°C),100mMKCl,100mM(NH4)2SO4,1%TritonX-100,1mg/mlBSA
- 10xPfuBufferwithMgSO4:200mMTris-HCl(pH8.8at25°C),100mMKCl,100mM(NH4)2SO4,1%TritonX-100,1mg/mlBSA,20mMMgSO4
- 25mMMgSO4Solution
Applications
- ClosetotentimesmoreaccuratethannormalDNApolymerase
- Producesblunt-endedamplificationproductstobeusedforcloning
- Remainsactiveevenafterincubating90minutesat95°C
StorageConditions
Storeat-20°C.Guaranteedstablefor12monthswhenproperlystored.
