Description
EpiQuikHotTaqischemicallymodifiedEpiQuikTaqDNAPolymerase.Theenzymeisinactiveatambienttemperature,havingnopolymeraseactivity.ToactivatetheEpiQuikHotTaqDNAPolymeraseitshouldbeincubatedat95-97°Cfor15minutesasafirstPCRstep.ThisenzymeallowsthePCRsetupatambienttemperaturewithoutnonspecificannealingandextension.PurifiedfromarecombinantE.colistrainwithclonedgeneencodingThermusaquaticusDNApolymerase.EpiQuikHotTaqDNAPolymerasehas5’→3’DNAsynthesisactivity.

Concentration
10units/µl(oneunitoftheenzymecatalyzestheincorporationof10nanomolesofdeoxyribonucleotidesintoapolynucleotidefractionin30minat70°C).

Source
RecombinantE.colistrainwithclonedgeneencodingThermusaquaticusDNApolymerase.

Composition

• EpiQuikHotTaqDNAPolymeraseinStorageBuffer:20mMTris-HCl(pH8.0),1mMDTT,0.1mMEDTA,100mMKCl,0.5%NonidetP40,0.5%Tween20and50%glycerol
• 10xReactionBuffer:100mMTris-HCl(pH8.8at25°C),500mMKCl,0.8%NonidetP40
• 10xReactionBufferwith(NH4)2SO4:Tris-HCl(pH8.8at25°C),200mM(NH4)2SO4,0.1%Tween20
• 25mMMgCl2Solution

Applications

• Polymerasechainreactionsetupatroomtemperature
• Effectiveincorporationofmodifiednucleotides

StorageConditions
Storeat-20°C.Guaranteedstablefor12monthswhenproperlystored.