Description
EpiQuikTaqDNAPolymeraseisarecombinantThermostableDNApolymerasethathasbeenpurifiedfromtheclonedThermusaquaticusDNApolymerasegeneexpressedinE.coli.EpiQuikTaqDNApolymerasehas5´→3´DNAsynthesisactivitybutlacksthe3´→5´exonucleaseactivity.

Concentration
5units/µl(oneunitoftheenzymecatalyzestheincorporationof10nanomolesofdeoxyribonucleotidesintoapolynucleotidefractionin30minat70°C).

Source
RecombinantE.colistrainwithclonedgeneencodingThermusaquaticusDNApolymerase.

Composition

• EpiQuikTaqDNAPolymeraseinStorageBuffer:20mMTris-HCl(pH8.0),1mMDTT,0.1mMEDTA,100mMKCl,0.5%NonidetP40,0.5%Tween20and50%glycerol
• 10xReactionBuffer:100mMTris-HCl(pH8.8at25°C),500mMKCl,0.8%NonidetP40
• 10xReactionBufferwith(NH4)2SO4:750mMTris-HCl(pH8.8at25°C),200mM(NH4)2SO4,0.1%Tween20
• 25mMMgCl2Solution

Applications

• Polymerasechainreaction(PCR) 
• Primerextension
• DNAsequencing

StorageConditions
Storeat-20°C. Guaranteedstablefor12monthswhenproperlystored.

Fig.1. Amplificationof420bptargetDNAusingEpiQuikTaqDNAPolymerase.

M–EpiQuik100bpDNALadder

1-3–EpiQuikTaqDNAPolymerase