| ExtractType: | DNA |
| OriginSpecies: | Human |
| 100%Guarantee: | 6months |
Description
ThishumanbraingenomicDNAishighlypurifiedandcarefullyisolatedfromhumanadultnormalbraintissues.EvidenceshowsthatDNAisolatedfromhumanbraintissuescontainshighamountsof5-hydroxymethylcytosine(5-hmC),amodifiedcytosinewhichplaysanimportantroleinepigeneticregulationofgenefunction.TheaveragelengthyoftheDNAisgreaterthan50kbandissuitableforgenomiclibraryconstructionandasacontrolDNAfor5-hmCdetection.A260/280isbetween1.8and2.0,andA260/230isabout2.0astestedbyspectrophotometer.
Concentration
200ng/μl
Size
10μgin50μl
StorageBuffer
10mMTris-HClpH8.0,1mMEDTA
QualityAssurance
PurifiedfreeofcontaminatingproteinandRNA.A260/280ratio:1.8,andA260/230ratio:~2.0
Storage
HumanbraingenomicDNAshouldbestoredat-20°Cforupto12months.Repeatedfreeze/thawcyclesshouldbeavoided.
ExtractOrigin
Humanadultnormalbraintissue
ResearchUse
Forresearchuseonly.
Applications
Genomiclibraryconstruction;Controlfor5-hmCdetection
