Description
ThishumanbraingenomicDNAishighlypurifiedandcarefullyisolatedfromhumanadultnormalbraintissues.EvidenceshowsthatDNAisolatedfromhumanbraintissuescontainshighamountsof5-hydroxymethylcytosine(5-hmC),amodifiedcytosinewhichplaysanimportantroleinepigeneticregulationofgenefunction.TheaveragelengthyoftheDNAisgreaterthan50kbandissuitableforgenomiclibraryconstructionandasacontrolDNAfor5-hmCdetection.A260/280isbetween1.8and2.0,andA260/230isabout2.0astestedbyspectrophotometer.

Concentration
200ng/μl

Size
10μgin50μl

StorageBuffer
10mMTris-HClpH8.0,1mMEDTA

QualityAssurance
PurifiedfreeofcontaminatingproteinandRNA.A260/280ratio:1.8,andA260/230ratio:~2.0

Storage
HumanbraingenomicDNAshouldbestoredat-20°Cforupto12months.Repeatedfreeze/thawcyclesshouldbeavoided.

ExtractOrigin
Humanadultnormalbraintissue

ResearchUse
Forresearchuseonly.

Applications
Genomiclibraryconstruction;Controlfor5-hmCdetection