| ExpressedIn: | E.Coli |
| ProteinSpecies: | Human |
| ResearchArea: | HistoneSumoylation |
| 100%Guarantee: | 6months |
Description
AllSUMOisoformsaretranslatedwithadditionalC-terminalresiduesthathavetoberemovedtogeneratetheactiveprotein.Pro-SUMO-3(103aminoacids)istheinactiveprecursorofSUMO-3(92aminoacids)andisprocessedattheC-terminusbySUMO-3specificproteases(SENPs).TheresultingSUMO-3proteinhastheconservedC-terminalGly-Glyresiduesthatfunctioninactivationandconjugationreactions.ThisproteincanbeusedasanegativecontrolinSUMOylationreactionsorasasubstrateforSENPs.
MolecularWeight(kDa)
11.6kDa
Formulation
50mMHEPESpH8.0,150mMNaCl,1mMDTT,10%glycerol
Source
ExpressedinE.coliexpressionsystem
Size
100µg
StorageTemp&Conditions
Storestocksolutionat-80°C.Avoidmultiplefreeze/thawcycles.
PurificationNotes
Purity:>90%
ApplicationNotes
Enzyme-linkedImmunoabsorbentAssay;WesternBlot(Recombinantprotein;ProteinArray;;Bestusewithinthreemonthsfromthedateofreceiptofthisprotein.
