| ExpressedIn: | E.Coli |
| ProteinSpecies: | Human |
| ResearchArea: | HistoneSumoylation |
| 100%Guarantee: | 6months |
Description
AllSUMOisoformsaretranslatedwithadditionalC-terminalresiduesthathavetoberemovedtogeneratetheactiveprotein.Pro-SUMO-2(95aminoacids)istheinactiveprecursorofSUMO-2(93aminoacids)andisprocessedattheC-terminusbySUMO-2specificproteases(SENPs).TheresultingSUMO-2proteinhastheconservedC-terminalGly-Glyresiduesthatfunctioninactivationandconjugationreactions.ThisproteincanbeusedasanegativecontrolinsumoylationreactionsorasasubstrateforSENPs.
MolecularWeight(kDa)
10.9kDa
Formulation
50mMHEPESpH8.0,150mMNaCl,1mMDTT,10%glycerol
Source
ExpressedinE.coliexpressionsystem
Size
100µg
StorageTemp&Conditions
Storeat-80°C.Avoidmultiplefreeze/thawcycles.
PurificationNotes
Purity:>95%bySDS-PAGE
ApplicationNotes
Typicalinvitroconcentrationsare10-50μMdependingonconditions
