| ExpressedIn: | E.Coli |
| ProteinSpecies: | Human |
| ResearchArea: | HistoneSumoylation |
| 100%Guarantee: | 6months |
Description
AllSUMOisoformsaretranslatedwithadditionalC-terminalresiduesthathavetoberemovedtogeneratetheactiveprotein.Pro-SUMO-1(101aminoacids)istheinactiveprecursorofSUMO-1(97aminoacids)andisprocessedattheC-terminusbySUMO-1specificproteases(SENPs).TheresultingSUMO-1proteinhastheconservedC-terminalGly-Glyresiduesthatfunctioninactivationandconjugationreactions.ThisproteincanbeusedasanegativecontrolinsumoylationreactionsorasasubstrateforSENPs.
MolecularWeight(kDa)
11.6kDa
Formulation
50mMHEPESpH8.0,150mMNaCl,1mMDTT,10%glycerol
Source
ExpressedinE.coliexpressionsystem
Size
100µg
StorageTemp&Conditions
Storeat-80°C.Avoidmultiplefreeze/thawcycles.Bestusewithinthreemonthsfromdateofreceipt.
PurificationNotes
Purity:>95%byHPLC
ApplicationNotes
Enzyme-linkedImmunoabsorbentAssay;WesternBlot(Recombinantprotein);AntibodyProduction;ProteinArray.
