Description
AURKBHumanisasingle,non-glycosylated,polypeptidechaincontaining364aminoacids(1-344..AURKBisfusedto20a.a.His-TagatN-terminusandpurifiedbyproprietarychromatographictechniques.AuroraKinaseB(AURKB)belongstoafamilyofmitoticserine/threoninekinases.AURKBconnectswithchromosomesfortheperiodofprophasepriortorelocalizingtothespindleatanaphase.AURKBlocalizestomicrotubulesnearkinetochores,specificallytothespecializedmicrotubulescalledK-fibers.AURKBcontrolschromosomesegregationthroughthecontrolofmicrotubule-kinetochoreattachmentandcytokinesis.AURKBisrequiredforkinetochorelocalizationofBUB1andSGOL1.AURKBexpressionduringtheG2/MphasetransitionisfirmlycoordinatedwithhistoneH3phosphorylation,whileoverexpressionisseeninmanykindsofcancers.AURKBphosphorylates"Ser-10"and"Ser-28"ofhistoneH3duringmitosis.AURKBisacomponentoftheCPC(chromosomalpassengercomplex),whichisacomplexthatactsasakeyregulatorofmitosis.HighlevelexpressionofAURKBisseeninthethymus,whichisalsoexpressedinthespleen,lung,testis,colon,placentaandfetalliver.AURKBisexpressedduringSandG2/Mphaseandexpressionisup-regulatedincancercellsduringMphase.

MolecularWeight(kDa)
41.4kDa

Formulation
TheAURKBsolutioncontaining20mMTris-HClbuffer(pH8.0),0.5mMDTT,20%glycerol,0.1mMEDTA,0.1mMEGTA,0.1MNaCland0.1mMPMSF

Source
ExpressedinE.coliexpressionsystem

Size
10µg

StorageTemp&Conditions
Storebelow-18°C.Pleasepreventfreezethawcycles.Bestusewithinthreemonthsfromthedateofreceiptofthisprotein.

PurificationNotes
Purity:Greaterthan90.0%asdeterminedbySDS-PAGE.

ApplicationNotes
Enzyme-linkedImmunoabsorbentAssay;WesternBlot(Recombinantprotein);AntibodyProduction;ProteinArray.