Description
AURKAHumanisasingle,non-glycosylated,polypeptidechaincontaining423aminoacids(1-403).AURKAisfusedto20a.a.His-TagatN-terminusandpurifiedbyproprietarychromatographictechniques.AURKA(AuroraKinaseA)belongstothemitoticserine/threoninekinasesfamily.AURKAisacellcycle-regulatedkinasewhichmaybeinvolvedinmicrotubuleformationand/orstABIlizationatthespindlepoleduringchromosomesegregation.AURKAisfoundatthecentrosomeininterphasecellsandatthespindlepolesinmitosis.SincetheAURKAexpressioniscell-cycleregulated,itislowinG1/S,itaccumulatesduringG2/M,anditdecreasesrapidlyafter.AURKAisinvolvedinimportantprocessesduringmitosisandmeiosiswhoseproperfunctionisessentialforhealthycellproliferation.Inaddition,AURKAplaysanessentialroleintumourigenesisandisoverexpressedinvarioustypesofcancers.AURKAisstronglyexpressedinthetestis,colon,ovarian,prostate,neuroblastoma,breastandcervicalcancercelllinesandweaklyinskeletalmuscle,thymusandspleen.AURKAinteractswithitssubstratesBORAandARHGEF2,aswellaswithTACC1andCPEB1.DefectsintheAURKAgenecausenumericalcentrosomeaberrationsincludinganeuploidy.AURKAoverexpressionhasbeenlinkedtochromosomalinstabilityincolorectalcancer.AURKAexpressionmayhaveaprognosticsignificanceinovariancarcinoma.

MolecularWeight(kDa)
47.9kDa

Formulation
TheAURKAsolutioncontaining20mMTris-HClbuffer(pH8.0),0.5mMDTT,100mMNaCl,0.1mMEDTA,0.1mMEGTA,0.1mMPMSFand20%glycerol.

Source
ExpressedinE.coliexpressionsystem

Size
10µg

StorageTemp&Conditions
Storebelow-18°C.Pleasepreventfreezethawcycles

PurificationNotes
Purity:Greaterthan95.0%asdeterminedbySDS-PAGE

ApplicationNotes
Enzyme-linkedImmunoabsorbentAssay;WesternBlot(Recombinantprotein);AntibodyProduction;ProteinArray;PreparationMethod:invitrowheatgermexpressionsystem;Bestusewithinthreemonthsfromthedateofreceiptofthisprotein