| ExpressedIn: | sf9Cells |
| ProteinSpecies: | Human |
| ResearchArea: | DNAMethylation |
| 100%Guarantee: | 6months |
Description
HumanCXXC6partialORF(NP_085128.1,1418a.a.-2136a.a.)recombinantproteinwithFLAG-tagatN-terminal.
MolecularWeight(kDa)
80 kDa
Formulation
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,20%glycerolintheelutionbuffer.
Source
PurifiedfromBaculovirusinfectedsf9cellexpressionsystem.
Concentration/Volume
10µg
StorageTemp&Conditions
Storeat-80°C.Aliquottoavoidrepeatedfreezingandthawing.Bestwhenusedwithinsixmonthsfromthedateofreceipt.
PurificationNotes
>80%purity
ApplicationNotes
ActiveDNAdemethylasecanbeusedforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
ActivityAssay
ActivityofrecombinantTET1proteinwasmeasuredwiththe Epigenase5mC-HydroxylaseTETActivity/InhibitionAssayKit(Fluorometric). Inthisassay,aTETsubstrateisconvertedtohydroxymethylatedproducts,whichisrecognizedwithaspecificantibody.Theratiooramountofhydroxymethylatedproductsisproportionaltoenzymeactivityandcanthenbefluorometricallymeasuredat530ex/590em.TheactivityoftheTETenzymeisinturnproportionaltotherelativefluorescentunitsmeasured.
AlternativeNames
Ten-eleventranslocation1protein(TET1),methylcytosinedioxygenaseTET1,LeukemiaassociatedproteinwithaCXXCdomain,CXXC-typezincfingerprotein6.
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