Background Apolyhistidine-tagisanaminoacidmotifinproteinsthatconsistsofatleastsixhistidine(His)residues,oftenattheN-orC-terminusoftheprotein.Polyhistidine-tagsareoftenusedforaffinitypurificationofpolyhistidine-taggedrecombinantproteinsthatareexpressedinEscherichiacoliorotherprokaryoticexpressionsystems. Description RabbitpolyclonalantibodytoHis-tag,HRPconjugated Purification Affinitypurified Specificity SpeciesIndependent Isotype IgG Immunogen ThisantibodyisproducedbyimmunizingrabbitswithasyntheticpeptideCHHHHHH(KLH-coupled). Formulation Liguid.1*TBS(pH7.4),0.5%BSA,25%Glycerol.Preservative:0.05%SodiumAzide. Storage Storeat4°Cafterthawing.Aliquotandstoreat-20°Cor-80°C.Avoidrepeatedfreeze/thawcycles. AlternativeNames 6Hisepitopetag,H,HIS,Histag,HHHHHHepitopetag,HHHHHHtag,Poly-histidine,His-TagPolyclonalAntibody,AntiHis-TagHRPConjugated Application WB:1:2,000 JHB0002 |
Fig.1. WesternblotanalysisonHis-Tagrecombinantprotein(26kDa)usingHis-TagPolyclonalTagAntibody,HRPConjugated.
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