| Application: | ELISA,IH,WB |
| AreasofResearch: | OtherAntibodies |
| Clonality: | Polyclonal |
| Conjugate: | KLH |
| Host: | Rabbit |
| Isotype: | IgG |
| Purification: | AffinityPurified |
| Reactivity: | Viral |
| 100%Guarantee: | 6months |
Description
HA-TagTagAntibodyisspecificfortheHA(Haemagglutinin)epitopetagpeptidesequenceYPYDVPDYA,whenfusedtoeithertheamino-orcarboxyl-terminusoftargetproteins,includingexpressioninmanyfrequentlyusedexpressionvectors.HA-TAGTagAntibodyhasbeentestedagainstboththeimmunogenandrecombinantproteinscontainingtheHAsequenceinELISAandWesternblotting.InWesternblottingofbacterialextractsthisantibodyhasbeenshownnottocross-reactwithanyendogenousproteins.
AntibodyType
RabbitPolyclonalAntibody
Purification
PurifiedIgG-liquid
Immunogen
KeyholelimpethemocyaninconjugatedepitopetagpeptideYPYDVPDYA(114-122)fromhaemagglutinininfluenza.AcysteineresiduewasusedtofacilitatecouplingattheC-terminalend.
Isotype
PolyclonalIgG
Size
50µg
Formulation
Phosphatebufferedsaline.0.09%SodiumAzide(NaN3)
Specificity
HA
Storage
Storeat+4ºCorat-20ºCifpreferred.Storageinfrost-freefreezersisnotrecommended.Thisproductshouldbestoredundiluted.Avoidrepeatedfreezingandthawingasthismaydenaturetheantibody.
HandlingRecommendations
Shouldthisproductcontainaprecipitatewerecommendmicrocentrifugationbeforeuse. Formaximumrecoveryoftheproducts,centrifugethevialpriortoopeningthecap.
Applications&SuggestedDilutions
ELISA;Immunohistology-Paraffin(Thisproductrequiresantigenretrievalusingheattreatmentpriortostainingofparaffinembeddedsections.Citratebufferisrecommendedforthispurpose);WesternBlotting
