Background
ThepGEX.3XexpressionvectordevelopedbySmithandJohnsonallowsforsynthesisoffusionproteinsbetweenglutathionine-S-transferase(GST)andproteinsencodedbyinsertedCDNAsequences.AntibodiesderivedfromtheseGSTfusionproteinsareusefulforcheckingproteinexpressionbothinplaquesandonWesternblots,aswellasforimmunogenaffinitypurificationofproteinsexpressedinE.coli. Description
RabbitpolyclonalantibodytoGST,HRPconjugated Purification
Affinitypurified Specificity
SpeciesIndependent Isotype
IgG Immunogen
ThisantibodyisproducedbyimmunizingrabbitswithGSTfusionproteinexpressedbypGEX-vector. Formulation
Liguid.1*TBS(pH7.4),0.5%BSA,40%Glycerol. Storage
Storeat4°Cafterthawing.Aliquotandstoreat-20°Cor-80°C. Avoidrepeatedfreeze/thawcycles. AlternativeNames
GSTAntibody,Glutathione-S-Transferase,HRPGSTantibody,GSTHRPConjugated,AntiGSTHRPConjugate Application
WB:1:5,000-1:10,000, ELISA:1:10,000 JHB0002 | 
 Fig.1. WesternblotanalysisonGSTfusionprotein(26kDa)using GSTPolyclonalTagAntibody,HRPConjugated.
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