Background
Non-receptortyrosine-proteinkinasethatplaysaroleinmanykeyprocesseslinkedtocellgrowthandsurvivalsuchasCytoskeletonremodelinginresponsetoextracellularstimuli,cellmotilityandadhesion,receptorendocytosis,autophagy,DNAdamageresponseandapoptosis.CoordinatesactinremodelingthroughtyrosinephosphorylationofproteinscontrollingcytoskeletondynamicslikeWASF3(involvedinbranchformation);ANXA1(involvedinmembraneanchoring);DBN1,DBNL,CTTN,RAPH1andENAH(involvedinsignaling);orMAPTandPXN(microtubule-bindingproteins).PhosphorylationofWASF3iscriticalforthestimulationoflamellipodiaformationandcellmigration.InvolvedintheregulationofcelladhesionandmotilitythroughphosphorylationofkeyregulatorsoftheseprocessessuchasBCAR1,CRK,CRKL,DOK1,EFSorNEDD9.PhosphorylatesmultiplereceptortyrosinekinasesandmoreparticularlypromotesendocytosisofEGFR,facilitatestheformationofneuromuscularsynapsesthrouhghMUSK,inhibitsPDGFRB-mediatedchemotaxisandmodulatestheendocytosisofactivatedBcellreceptorcomplexes.Othersubstrateswhichareinvolvedinendocytosisregulationarethecaveolin(CAV1)andRIN1.Moreover,ABL1regulatestheCBLfamilyofubiquitinligasesthatdrivereceptordown-regulationandactinremodeling.PhosphorylationofCBLleadstoincreasedEGFRstABIlity.Involvedinlate-stageautophagybyregulatingpositivelythetraffickingandfunctionoflysosomalcomponents.ABL1targetstomitochondriainresponsetooxidativestressandtherebymediatesmitochondrialdysfunctionandcelldeath.ABL1isalsotranslocatedinthenucleuswhereithasDNA-bindingactivityandisinvolvedinDNA-damageresponseandapoptosis.ManysubstratesareknownmediatorsofDNArepair:DDB1,DDB2,ERCC3,ERCC6,RAD9A,RAD51,RAD52orWRN.ActivatestheproapoptoticpathwaywhentheDNAdamageistooseveretoberepaired.PhosphorylatesTP73,aprimaryregulatorforthistypeofdamage-inducedappoptosis.PhosphorylatesPSMA7thatleadstoaninhibitionofproteasomalactivityandcellcycletransitionblocks.ABL1actsalsoasaregulatorofmultiplepathologicalsignalingcascadesduringinfection.Severalknowntyrosine-phosphorylatedmicrobialproteinshavebeenidentifiedasABL1substrates.ThisisthecaseofA36RofVacciniavirus,Tir(translocatedintiminreceptor)ofpathogenicE.coliandpossIBLyCitrobacter,CagA(cytotoxin-associatedgeneA)ofH.pylori,orAnkA(ankyrinrepeat-containingproteinA)ofA.phagocytophilum.PathogenscanhighjackABL1kinasesignalingtoreorganizethehostactincytoskeletonformultiplepurposes,likefacilitatingintracellularmovementandhostcellexit.Finally,functionsasitsownregulatorthroughautocatalyticactivityaswellasthroughphosphorylationofitsinhibitor,ABI1.

Description
ABL1PolyclonalAntibody,BiotinConjugated.Biotin.Raisedin:Rabbit.

Formulation
Liquid.0.03%Proclin300,50%Glycerol,0.01MPBS,PH7.4.

Specificity
Human

Isotype
IgG

UniprotID
P00519

Purification
>95%,ProteinGpurified

Immunogen
RecombinanthumanTyrosine-proteinkinaseABL1protein(4-194AA)

Storage
Shippedat4°C.Upondeliveryaliquotandstoreat-20°C(short-term)or-80°C(long-term).Avoidrepeatedfreeze.

Application
ELISA

VCU0006