| Application: | ELISA,IH |
| AreasofResearch: | DNADamage&Repair |
| Clonality: | Polyclonal |
| Conjugate: | Unconjugated |
| Host: | Goat |
| Isotype: | IgG |
| Purification: | Serum |
| Reactivity: | BroadRange |
| 100%Guarantee: | 6months |
Description
Epigentek"s8-hydroxyguanosineantibodyrecognizes8-hydroxyguanosine(8-OHG),amodifiedbasewhichoccursinDNAasaresultofoxidativestress.8-hydroxy-2"-deoxyguanosine(8-OHdG)hasbecomeasensitiveMarkerofoxidativedamageincellularDNAandhasbeenreportedtobeexcretedintheurine.Levelsof8-OHdGhavebeenshowntoincreaseonexposuretoionizingrADIationandtobaccosmoke.Epigentek"s8-hydroxyguanosinepolyclonalantibodycrossreactscompletelywith8-OHdG,anddoesnotcrossreactwithothernaturallyoccurringnucleotides.
AntibodyType
GoatPolyclonal
Purification
Serum-liquid
Immunogen
8-Hydroxyguanosine-conjugate
Isotype
PolyclonalIgG
Size
0.1ml
Formulation
0.1%SodiumAzide
Specificity
Chemical
Storage
Storeat+4°Corat-20°Cifpreferred.Thisproductshouldbestoredundiluted.Storageinfrostfreefreezersisnotrecommended.Avoidrepeatedfreezingandthawingasthismaydenaturetheprotein.
HandlingRecommendations
Shouldthisproductcontainaprecipitatewerecommendmicrocentrifugationbeforeuse.Formaximumrecoveryoftheproducts,centrifugethevialpriortoopeningthecap.
AlternativeNames
8hydroxy2’deoxyguanosine,8hydroxyguanine,8hydroxyguanosine,8OHG,8-OHG,8OG,8OHdG,8OHG
Applications&SuggestedDilutions
ELISA:1/100,000-1/250,000;Immunohistology-Paraffin:1/200;Wherethisantibodyhasnotbeentestedforuseinaparticulartechniquethisdoesnotnecessarilyexcludeitsuseinsuchprocedures.Suggestedworkingdilutionsaregivenasaguideonly.Itisrecommendedthattheusertitratestheantibodyforuseintheirownsystemusingappropriatenegative/positivecontrols.
