>
产品中心 >
acid_base_buffer_solution >
Epigentek/5-Methylcytosine (5-mC) Monoclonal Antibody [33D3]/A-1014-010/10 µg
![Epigentek/5-Methylcytosine (5-mC) Monoclonal Antibody [33D3]/A-1014-010/10 µg](https://www.ebiomall.cn/images/Epigentek/201710/a1014data1.gif)
| Application: | DB,ELISA,IF,IHC,IP,MeDIP |
| AreasofResearch: | DNAMethylation |
| Clonality: | Monoclonal |
| Conjugate: | Unconjugated |
| Host: | Mouse |
| Isotype: | IgG1 |
| Purification: | AffinityPurified |
| Reactivity: | BroadRange |
| 100%Guarantee: | 6months |
Background
5-methylcytosine(5-mC)isformedwhenDNAmethyltransferase(DNMT)catalyzestheadditionofamethylgroupontothe5-carbonofthecytosinering,anepigeneticprocessknownas DNAmethylation.5-mCisconsideredthe"fifth"DNAbaseandthis5-methylcytosinemousemonoclonalantibodyisidealfordiscriminatingbetweentheunmodifiedcytosinebase(C)andthemethylatedcytosinebase(5-mC)forDNAmethylationstudies.DNAmethylation,themajorepigeneticmodificationofeukaryoticgenomes,playsanessentialroleinmammaliandevelopment.DNAmethylationofpromoterregionsleadstoinactivationofgenefunction.Also,DNAmethylationstatusvariesaccordingtotissuetype,andregion-specificDNAhypermethylationandglobalDNAhypomethylationhavebeendemonstratedtoplayanimportantroleintumOrigenesis.
Description
Mousemonoclonalantibodyto5-methylcytosine(5-mC),clone33D3,MeDIP/ChIP-grade,usedinDNAmethylationstudies.
Concentration
1mg/ml
Purification
ProteinA
Immunogen
Ovalbumin-conjugated5-methylcytosine(5-mC)
Isotype
IgG1
Specificity
Modifiedbase5-methylcytosine(5-mC),abroadrangeofspecies.
Formulation
PurifiedIgGin10mMphosphatebuffer,150mMNaCl,pH7.4.
Storage
4°C,stablefor6monthsfromthedateofshipment.Forlong-termstorage,aliquotandstoreat-20°C.Avoidrepeatedfreezingandthawing.Multiplefreeze/thawcyclesmayresultindecreasedperformance.
HandlingRecommendations
Formaximumrecoveryoftheproducts,centrifugethevialpriortoopeningthecap.
AlternativeNames
5-methylcytidine,anti-5-methylcytidine,anti-5-methylcytosine,anti-5mC,anti-5-mC,anti-5meC,anti-5-meC,5mC,5meC,5-meC, 5"-methyl-2"-deoxycytidine,5MedCyd
Application&SuggestedDilutions*
DotBlot:1:1000-1:2000;Immunohistochemistry:1:100-1:500;Immunofluorescence:1:100-1:500;ELISA:1:1000-1:2000;MeDIP:0.5-1µg/reaction
*TheenduserisresponsIBLefordeterminingoptimalworkingdilutions.
