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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > Epigentek/EpiQuik全局组蛋白H3磷酸化(Ser28)分析试剂盒(荧光法)/P-7005-48/48分析
商品详细Epigentek/EpiQuik全局组蛋白H3磷酸化(Ser28)分析试剂盒(荧光法)/P-7005-48/48分析
Epigentek/EpiQuik全局组蛋白H3磷酸化(Ser28)分析试剂盒(荧光法)/P-7005-48/48分析
Epigentek/EpiQuik全局组蛋白H3磷酸化(Ser28)分析试剂盒(荧光法)/P-7005-48/48分析
商品编号: P-7005-48
市场价: ¥5640.00
美元价: 3384.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
InputType:CellExtracts,HistoneExtracts,UnisolatedSamples
ResearchArea:Phosphorylation
TargetApplication:AmountQuantitation
VesselFormat:96-WellPlate
100%Guarantee:6months
ProductOverview

TheEpiQuik™GlobalHistoneH3Phosphorylation(Ser28)AssayKit(Fluorometric)isaconvenientsetoftoolsthatallowstheexperimentertomeasureglobalphosphohistoneH3(ser28).Thekitisready-to-useandprovidesalltheessentialcomponentsneededforspecificallymeasuringglobalhistoneH3phophorylationatser28usingavarietyofmammaliancells(human,mouse,etc.)includingfreshandfrozentissues,andculturedadherentandsUSPensioncells.Thekithasthefollowingadvantages:

  • Quickandefficientprocedure,whichcanbefinishedwithin3hours.
  • InnovativefluorometricassaywithouttheneedforrADIoactivity,electrophoresis,orchromatography.
  • SpecificallycapturesphosphohistoneH3(ser28)withthedetectionlimitaslowas0.5ng/well.
  • ThecontrolisconvenientlyincludedforthequantificationofphosphorylatedhistoneH3(ser28).
  • StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughput.
  • Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™GlobalHistoneH3Phosphorylation(Ser28)AssayKit(Fluorometric)isdesignedformeasuringglobalhistoneH3phosphorylationatser28.Inanassaywiththiskit,thephosphorylatedhistoneH3atser28iscapturedtothestripwellscoatedwithananti-phosphohistoneH3(ser28)antibody.ThecapturedphosphohistoneH3(ser28)canthenbedetectedwithalabeleddetectionantibody,followedbyafluorodevelopmentreagent.TheratioofphosphohistoneH3(ser28)isproportionaltotheintensityoffluorescence.TheabsoluteamountofphosphohistoneH3(ser28)canbequantitatedbycomparingtothestandardcontrol.

 
Fig.1.SchematicprocedureforusingtheEpiQuikGlobalHistoneH3Phosphorylation(Ser28)AssayKit(Fluorometric).

ProductComponents

F1(10XWashBuffer)
F2(AntibodyBuffer)
F3(DetectionAntibody,1mg/ml)*
F4(FluoroDeveloper)*
F5(FluoroEnhancer)*
F6(FluoroDilution)
StandardControl(100µg/ml)*
8-WellSampleStrips(withFrame)
8-WellStandardControlStrips
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialpriortoopeningthecap.

品牌介绍
Epigentek快速,有效地制备DNA,蛋白质和染色质对于进行下游分析至关重要。我们的样品制备套件套件几乎适用于所有工作流程,无论是为ChIP-Seq或ChIP-PCR应用分离染色质,还是从各种起始原料制备亚硫酸氢盐-Seq或MS-PCR的DNA。我们的样品制备套件使您可以直接从提取到反应,为您的实验工作流程提供无数种可能性和灵活性。我们的DNA,蛋白质和染色质制备试剂盒是无数研究机会和下游应用的起点。