Epigentek/EpiQuik原位组蛋白H3磷酸化（Ser10）分析试剂盒/P-7001-96/96分析-免疫在线蚂蚁淘旗下平台

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商品详细Epigentek/EpiQuik原位组蛋白H3磷酸化（Ser10）分析试剂盒/P-7001-96/96分析

Epigentek/EpiQuik原位组蛋白H3磷酸化（Ser10）分析试剂盒/P-7001-96/96分析

商品编号： P-7001-96

品牌： Epigentek Group Inc

市场价： ¥9380.00

美元价： 5628.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

InputType:	Cells
ResearchArea:	Phosphorylation
TargetApplication:	AmountQuantitation
VesselFormat:	96-WellPlate
100%Guarantee:	6months

ProductOverview

TheEpiQuik™InSituHistoneH3Phosphorylation(Ser10)AssayKitisaconvenientsetoftoolsthatallowstheexperimentertomeasureinsituphosphohistoneH3(ser10).Thekitisready-to-useandprovidesalltheessentialcomponentsneededforspecificallymeasuringhistoneH3phosphorylation(ser10)insituusingculturedadherentcells.Thekithasthefollowingadvantages:

Quickandefficientprocedure,whichcanbefinishedwithin3hours.
InnovativecolorimetricassaywithouttheneedforrADIoactivity,electrophoresis,orchromatography.
MeasurementofinsituhistoneH3phosphorylationatser10andwithouttheneedtopreparecelllysates.
MicroplateformatmakestheassaysuitableforhighthroughputanalysisofreagentsthatincreasesorinhibitshistoneH3phosphorylationatser10.
Simple,reliable,andconsistentassayconditions.

Principle&Procedure
EpiQuik™InSituHistoneH3Phosphorylation(Ser10)AssayKitisawholecell-baseddetectionkitofhistoneH3phosphorylation(ser10).Inthisassay,adherentcellsareculturedinconventional96-wellmicroplates.Afteryourexperimentaltreatment,cellsarefixedandpermeABIlized.ThehistoneH3phosphorylation(ser10)isthendetectedbyananti-phosphohistoneH3(ser10)antibody.TheratiooramountofphosphohistoneH3(ser10)canbequantifiedthroughHRPconjugatedsecondaryantibody-colordevelopmentsystemandisproportionaltotheintensityofcolordevelopment.

Fig.1.SchematicprocedureforusingtheEpiQuikInSituHistoneH3Phosphorylation(Ser10)AssayKit.

Fig.2. Log-phaseMCF-7cellswereserum-starvedovernight,andserumwasaddedbackfor30minutesfollowedbytreatmentwithCalyculinA(CL-A,0.2Î¼Mfor30minutes).ThelevelofH3phosphorylationatser10wasmeasuredwiththiskit.

ProductComponents

PA1(10XWashBuffer)
PA2(PermeabilizingBuffer)
PA3(BlockingBuffer)
PA4(AntibodyBuffer)
PA5(CaptureAntibody,1000µg/ml)*
PA6(DetectionAntibody,200µg/ml)*
PA7(DevelopingSolution)
PA8(StopSolution)
30%H2O2Solution
PhosphoH3^Ser10Control(20µg/ml)
8-WellControlStrips
Microplates
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialpriortoopeningthecap.

品牌介绍

Epigentek快速，有效地制备DNA，蛋白质和染色质对于进行下游分析至关重要。我们的样品制备套件套件几乎适用于所有工作流程，无论是为ChIP-Seq或ChIP-PCR应用分离染色质，还是从各种起始原料制备亚硫酸氢盐-Seq或MS-PCR的DNA。我们的样品制备套件使您可以直接从提取到反应，为您的实验工作流程提供无数种可能性和灵活性。我们的DNA，蛋白质和染色质制备试剂盒是无数研究机会和下游应用的起点。

公司介绍

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服务热线：4000-520-616

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手机：18915418616

官网：http://www.epigentek.com