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Epigentek/EpiQuik循环组蛋白H3瓜氨酸化ELISA试剂盒（比色法）/P-3097-96/96分析
| InputType: | PlasmaorSerum |
| ResearchArea: | HistoneCitrullination |
| TargetApplication: | AmountQuantitation |
| VesselFormat: | 96-WellPlate |
| 100%Guarantee: | 6months |
TheEpiQuik™CirculatingHistoneH3CitrullinationELISAKit(Colorimetric) isacompletesetofoptimizedbuffersandreagentsspecificallydesignedtoquantitativelydetectcirculatinghistoneH3citfromplasmaandserum.Thiskithasthefollowingadvantages:
- Quickandefficientprocedure,whichcanbefinishedwithin2.5hours.
- Highsensitivityandspecificity.Thedetectionlimitisaslowas0.5ng/wellwithdynamicrangeof1-20ng/wellwithintheindicatedamountrangeoftheplasma/serum.OnlyrecognizesH3citwithnocross-reactivitywithunmodifiedH3orothermodificationsatthesameargininesite.
- ThecontrolisconvenientlyincludedforthequantificationofH3cit.
- StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughput.
- Simple,reliable,andconsistentassayconditions.
BackgroundInformation
Argininehistonecitrullinationisoneofthemanyimportantepigeneticmarks,andisessentialfortheregulationofepigeneticchromatinremodelingandimmunecells’extracellulartrapprocesses.CitrullinatedarginineofhistoneH3(Arg2,8,17)regulatesgeneexpressionandismainlycatalyzedbypeptidylargininedeiminase4(PAD4). FurThermore,citrullinationofhistones,inparticularhistoneH3,wasrevealedasaconvergencepointfordiverseinflammatorysignalsthattriggertheneutrophilresponsetoinfections.ItwasreportedthatcitrullinatedhistoneH3couldbeapotentialserumbioMarkerfortheearlydiagnosisofsepticshock.MoreimmunologicaldiseasessuchasmultiplesclerosisandrheumatoidarthritisseemtobealsoassociatedwithchangeofhistoneH3citrullination. ThecirculatinghistoneH3citrullinationcanbechangedbyinhibitionoractivationofPADs.Therefore,quantitativedetectionofcirculatinghistoneH3citrullinationwouldprovideusefulinformationforbetterunderstandingepigeneticregulationofgeneactivationandsilencing,aswellasfordevelopingPAD-targeteddrugs.
Principle&Procedure
ThiskitisdesignedformeasuringtotalH3citinplasmaorserum.Inanassaywiththiskit,thecitrullinatedhistoneproteinsintheplasma/serumsamplearecapturedonthestripwellscoatedwithanti-citrullinatedH3antibody.ThecapturedcitrullinatedH3canthenberecognizedwithadetectionantibodyfollowedbyacolordevelopmentreagent.TheratioofH3citisproportionaltotheintensityofabsorbance.TheabsoluteamountofH3citcanbequantitatedbycomparingtothestandardcontrol.
StartingMaterials
Inputmaterialsshouldbeplasmaorserum.Theamountofplasmaorserumforeachassaycanbe10to40µlwithanoptimalamountof30µl.
WB(10XWashBuffer)
HAB(HistoneAssayBuffer)
DS(DeveloperSolution)
SS(StopSolution)
8-WellAssayStrips(WithFrame)
ControlAssayStrips(WithFrame)#
AdhesiveCoveringFilmDAb(DetectionAntibody,1000X)*
StandardControl(100µg/ml)
*Spinthesolutiondowntothebottompriortouse.
#ControlAssayStripsaregreentrimmedfordistinguishingfrom8-wellAssayStrips(forsamples).
TheControlAssayStripsareonlyforcontroluseandshouldnotbeusedforsampleassay.
