TheEpiQuik™GlobalHistoneH4AcetylationAssayKit isaconvenientpackageoftoolsthatallowstheexperimentertomeasureglobalacetylationofhistoneH4attremendouslyfastspeedsandconsistency,superiorandsaferthanallothercurrentmethods.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulassayexperiment.TheEpiQuik™GlobalHistoneH4AcetylationAssayKitissuitableforspecificallymeasuringglobalhistoneH4acetylationusingavarietyofmammaliancellsincludingfreshandfrozentissues,andculturedadherentandsUSPensioncells.Thekithasthefollowingadvantages:

• Quickandefficientprocedure,whichcanbefinishedwithin5hours.
• InnovativecolorimetricassaywithouttheneedforrADIoactivity,electrophoresis,orchromatography.
• StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
• Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™GlobalHistoneH4AcetylationAssayKitisdesignedformeasuringglobalhistoneH4acetylation.Inanassaywiththiskit,thehistoneproteinsarestablyspottedonthestripwells.TheacetylatedhistoneH4canberecognizedwithahigh-affinityantibody.TheratiooramountofacetylatedH4canbequantifiedthroughHRPconjugatedsecondaryantibody-colordevelopmentsystemandisproportionaltotheintensityofcolordevelopment.

GE1(10Xlysisbuffer)
GE2(extractionbuffer)
GE3(10Xwashbuffer)
GE4(histonebuffer)
GE5(blockingbuffer)
GE6(antibodybuffer)
GE7(captureantibody,100µg/ml)*
GE8(detectionantibody,400µg/ml)*
GE9(developingsolution)
GE10(stopsolution)
AcetylatedhistoneH4control(60µg/ml)*
8wellassaystrips(withframe)
Userguide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialpriortoopeningthecap.