TheEpiQuik™GlobalHistoneH3AcetylationAssayKit isaconvenientpackageoftoolsthatallowstheexperimentertomeasureglobalacetylationofhistoneH3attremendouslyfastspeedsandconsistency,superiorandsaferthanallothercurrentmethods.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulassayexperiment.TheEpiQuik™GlobalHistoneH3AcetylationAssayKitissuitableforspecificallymeasuringglobalhistoneH3acetylationusingavarietyofmammaliancellsincludingfreshandfrozentissues,andculturedadherentandsUSPensioncells.Thekithasthefollowingadvantages:

• Quickandefficientprocedure,whichcanbefinishedwithin5hours.
• InnovativecolorimetricassaywithouttheneedforrADIoactivity,electrophoresis,orchromatography.
• StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
• Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™GlobalHistoneH3AcetylationAssayKitisdesignedformeasuringglobalhistoneH3acetylation.Inanassaywiththiskit,thehistoneproteinsarestablyspottedonthestripwells.TheacetylatedhistoneH3canberecognizedwithahigh-affinityantibody.TheratiooramountofacetylatedhistoneH3canbequantifiedthroughHRPconjugatedsecondaryantibody-colordevelopmentsystemandisproportionaltotheintensityofcolordevelopment.

GF1(10Xlysisbuffer)
GF2(extractionbuffer)
GF3(10XWashBuffer)
GF4(HistoneBuffer)
GF5(BlockingBuffer)
GF6(AntibodyBuffer)
GF7(CaptureAntibody,100µg/ml)*
GF8(DetectionAntibody,400µg/ml)*
GF9(DevelopingSolution)
GF10(StopSolution)
AcetylatedHistoneH3Control(60µg/ml)*
8-WellAssayStrips(withFrame)
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialpriortoopeningthecap.