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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > Epigentek/EpiQuik HDAC2 Assay Kit (Colorimetric)/P-4006-96/96 assays
商品详细Epigentek/EpiQuik HDAC2 Assay Kit (Colorimetric)/P-4006-96/96 assays
Epigentek/EpiQuik HDAC2 Assay Kit (Colorimetric)/P-4006-96/96 assays
Epigentek/EpiQuik HDAC2 Assay Kit (Colorimetric)/P-4006-96/96 assays
商品编号: P-4006-96
市场价: ¥11180.00
美元价: 6708.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
InputType:NuclearExtracts,PurifiedEnzyme
ResearchArea:HistoneAcetylation
TargetApplication:AmountQuantitation
VesselFormat:96-WellPlate
100%Guarantee:6months
ProductOverview

TheEpiQuik™HDAC2AssayKitisaconvenientpackageoftoolsthatallowstheexperimentertomeasuretheamountofHDAC2attremendouslyfastspeedsandconsistency;superiorandsaferthanallothercurrentmethods.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulHDAC2assayexperimentwithouttheneedforelectrophoresisandtransferprocess.ItissuitableformeasuringHDAC2levelfromvariousfreshtissuesandculturedmammaliancells.Thekithasthefollowingadvantages:

  • Fastprocedure,whichcanbefinishedwithin3hours.
  • Innovativecolorimetricassaytosemi-quantitativelymeasureHDAC2amountwithouttheneedforelectrophoresis.
  • StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
  • Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™HDAC2AssayKitisdesignedformeasuringtotalHDAC2amountfromtissuesorcells.Inanassaywiththiskit,thenuclearproteinscontainingHDAC2arestablycoatedonthestripwells.TheHDAC2isrecognizedwitha high-affinityspecificantibody.TheamountofHDAC2canbequantifiedthrougha HRPconjugatedsecondaryantibody-colordevelopmentsystemandisproportionaltotheintensityofcolordevelopment.

 
Fig.1.SchematicprocedureforusingtheEpiQuikHDAC2AssayKit(Colorimetric).

Fig.2. HDAC2proteinswereboundtomicroplatewellsatdifferentconcentrationsandmeasuredusingtheEpiQuik™HDAC2AssayKit.

ProductComponents

HB1(10XWashBuffer)
HB2(HDACAssayBuffer)
HB3(BlockingBuffer)
HB4(CaptureAntibody,200µg/ml)*
HB5(DetectionAntibody,200µg/ml)*
HB6(DevelopingSolution)
HB7(StopSolution)
HDAC2Control(100ng/µl)
8-WellAssayStrip(withFrame)
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialafterthawingpriortoopeningthecap.

品牌介绍
Epigentek快速,有效地制备DNA,蛋白质和染色质对于进行下游分析至关重要。我们的样品制备套件套件几乎适用于所有工作流程,无论是为ChIP-Seq或ChIP-PCR应用分离染色质,还是从各种起始原料制备亚硫酸氢盐-Seq或MS-PCR的DNA。我们的样品制备套件使您可以直接从提取到反应,为您的实验工作流程提供无数种可能性和灵活性。我们的DNA,蛋白质和染色质制备试剂盒是无数研究机会和下游应用的起点。