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Epigentek/EpiQuik In Situ H3K9 Acetylation Assay Kit/P-4004-096/96 assays
| InputType: | Cells |
| ResearchArea: | HistoneAcetylation |
| TargetApplication: | AmountQuantitation |
| VesselFormat: | 96-WellPlate |
| 100%Guarantee: | 6months |
The EpiQuik™InSituH3-K9AcetylationAssayKit isaconvenientpackageoftoolsthatallowstheexperimentertomeasure insitu acetylationofhistoneH3-K9attremendouslyfastspeedsandconsistency,superiorandsaferthanallothercurrentmethods.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulassayexperiment.TheEpiQuik™ InSitu H3-K9AcetylationAssayKitissuitableforspecificallymeasuring insitu histoneH3-K9acetylationusingculturedadherentcells.Thekithasthefollowingadvantages:
- Quickandefficientprocedure,whichcanbefinishedwithin3hours.
- InnovativecolorimetricassaywithouttheneedforrADIoactivity,electrophoresis,orchromatography.
- Measurementof insitu histoneH3-K9acetylation,withouttheneedtopreparecelllysates.
- MicroplateformatmakestheassaysuitableforhighthroughputanalysisofagentsthatincreasesorinhibitsH3-K9acetylation.
- Simple,reliable,andconsistentassayconditions.
Principle&Procedure
EpiQuik™ InSitu HistoneH3-K9AcetylationAssayKituseswholecell-baseddetectionofacetylatedH3-K9.Inthisassay,adherentcellsareculturedinconventional96-wellmicroplates.Afteryourexperimentaltreatment,cellsarefixedandpermeABIlized.TheacetylatedH3-K9isthendetectedbyahighaffinityacetylH3-K9antibody.TheratiooramountofacetylH3-K9canbequantifiedthroughHRPconjugatedsecondaryantibody-colordevelopmentsystemandisproportionaltotheintensityofcolordevelopment.
GH1(10XWashBuffer)
GH2(PermeabilizingBuffer)
GH3(BlockingBuffer)
GH4(AntibodyBuffer)
GH5(CaptureAntibody,1mg/ml)*
GH6(DetectionAntibody,400µg/ml)*
GH7(DevelopingSolution)
GH8(StopSolution)
30%H2O2Solution
AcetylatedH3-K9Control(20µg/ml)
8-WellControlStrips
Microplates
UserGuide
*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialpriortoopeningthecap.
