TheEpiQuik™HATActivity/InhibitionAssayKitisaready-to-usesetofessentialcomponentsneededtomeasurehistoneacetyltransferaseactivity/inhibitionsafelyandquickly.Itissuitableformeasuringhistoneacetyltransferaseactivity/inhibitionfromabroadrangeofspeciesincludingmammaliancells/tissues,plants,andbacteria.Thekithasthefollowingadvantages:

• Veryquickprocedure,whichcanbecompletedwithinonly3hours.
• InnovativecolorimetricassaywithoutrADIoactivity,extraction,andchromatography.
• DirectmeasurementofHATactivityandinhibitionbyquantifyingtheamountofacetylatedhistonesubstrate,therebyavoidingthefalseinhibitoryeffectonHATs.
• StripmicroplateformatmakestheassayflexIBLeinbothmanualandhighthroughputanalysisformats.
• Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™HATActivity/InhibitionAssayKitisdesignedformeasuringtotalhistoneaceyltransferaseactivityorinhibition.Inanassaywiththiskit,theuniquehistonesubstrateisstablycapturedonthestripwells.ActiveHATsbindtoandacetylatehistonesubstrate.Theacetylatedsubstratecanberecognizedwithahighaffinityanti-acetylatedhistoneantibody.Theratiooramountoftheacetylatedhistone,whichisdirectlyproportionaltoHATenzymeactivity,canthen be colorimetricallyquantifiedthroughanELISA-likereaction.

 
Fig.1.SchematicprocedureforusingtheEpiQuikHATActivity/InhibitionAssayKit.

Fig.2. IllustratedstandardcurvegeneratedwithHATassaystandard.

Fig.3. NuclearproteinswerepreparedfromdifferentcelllinesandHATactivitywasmeasuredusingtheEpiQuik™HATActivity/InhibitionAssayKit.