InputType: | NuclearExtracts,PurifiedEnzyme |
ResearchArea: | DNAMethylation |
TargetApplication: | ActivityMeasurement |
VesselFormat: | 96-WellPlate |
100%Guarantee: | 6months |
TheEpigenase™ 5mC-HydroxylaseTETActivity/InhibitionAssayKit(Fluorometric)isacompletesetofoptimizedbuffersandreagentsformeasuringactivity/inhibitionoftotalcytosineoxygenaseTETenzymesinnuclearextractsorpurifiedTETisoforms(TETs1-3)fromabroadrangeofspeciessuchasmammals,plants,fungi,andbacteria,andinavarietyofformsincluding,butnotlimitedtoculturedcellsandfreshandfrozentissues. Thekithasthefollowingadvantagesandfeatures:
BackgroundInformation TheTETfamilyofcytosineoxygenasesincludesTET1,TET2,andTET3.TheseTETproteinsmaypromoteDNAdemethylationbybindingtoCpG-richregionstopreventunwantedDNAmethyltransferaseactivity,andbyconverting5mCto5hmC andfurtherto5caC(5-carboxylcytosine) throughhydroxylaseactivity.Itwasshownthatgenomic5hmClevelscorrelatewithTEThydroxylaseactivity.Inaddition, TET1wasshowntohavedualfunctionsintranscriptionactivationandrepressionbybindingdifferenttargetgenesinEScells.TET1isalsoafusionpartneroftheMLLgeneinacutemyeloidleukemiaandisconsideredanoncoprotein.TET2isfoundtobefrequentlymutatedinleukemiaandconsideredtoactastumorsuppressor.TET3wasdemonstratedtoplayauniqueroleinDNAmethylationreprogrammingprocessesinthemammalianzygote.Thus,activatingtumorsuppressorTETenzymessuchasTET2orinhibitingoncoproteinTETenzymessuchasTET1wouldbeimportantinbenefitingcancerdiagnositcsanddevelopingnewtarget-basedcancertherapeutics. Principle&Procedure | Fig.1. SchematicprocedurefortheEpigenase™5mC-HydroxylaseTETActivity/InhibitionAssayKit(Fluorometric).
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WBWashBuffer
TABTETAssayBuffer
TS10XTETSubstrate
BSBindingSolution
HC5TETAssayStandard
HC6CaptureAntibody
HC7DetectionAntibody
HC8EnhancerSolution
FDFluoroDeveloper
FEFluoro-Enhancer
Co-factor1
Co-factor2
Co-factor3
8-WellAssayStrips(WithFrame)