TheEpiQuik™Dnmt3BAssayKitisaconvenientpackageoftoolsthatallowstheexperimentertomeasuretheamountofDnmt3Battremendouslyfastspeedsandconsistency;superiorandsaferthanallothercurrentmethods.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulDnmt3Bassayexperiment.TheEpiQuik™Dnmt3BAssayKitissuitableformeasuringDnmtamountsquantitativelyfromfreshtissueandculturedcellsofhumanandmouse.Thekithasthefollowingadvantages:

• Thefastestprocedure,whichcanbefinishedwithin3hours.
• Innovativecolorimetricassay,whichquantitativelymeasureDnmt3Bamount withoutthe needforelectrophoresis.
• StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
• Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™Dnmt3BAssayKitisdesignedformeasuringtotalDnmt3Bamountfromtissuesorcells.Inanassaywiththiskit,theuniqueDnmtaffinitysubstrateisstablycoatedonthestripwell.ThesampleisaddedintothewellandDnmt3Bcontainedinthesamplebindstothesubstrate.TheboundDnmt3BcanberecognizedwithaspecificDnmt3BantibodyandcolorimetricallyquantifiedthroughanELISA-likereaction.TheamountofDnmt3Bisproportionaltotheintensityofcolordevelopment.

 
Fig.1.SchematicprocedureforusingtheEpiQuikDnmt3BAssayKit.

Fig.2. NuclearextractswerepreparedfromMCF-7cellsusingtheEpiQuikNuclearExtractionKitandtotalamountofDnmtwasmeasured.