TheEpiQuik™Dnmt3AAssayKitisaconvenientpackageoftoolsthatallowstheexperimentertomeasuretheamountofDnmt3Aattremendouslyfastspeedsandconsistency;superiorandsaferthanallothercurrentmethods.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulDnmt3Aassayexperiment.TheEpiQuik™Dnmt3AAssayKitissuitableformeasuringDnmtamountsquantitativelyfromfreshtissueandculturedcellsofhumanandmouse.Thekithasthefollowingadvantages:

• Thefastestprocedure,whichcanbefinishedwithin3hours.
• Innovativecolorimetricassay,whichquantitativelymeasureDnmt3Aamountwithoutthe needforelectrophoresis.
• StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
• Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™Dnmt3AAssayKitisdesignedformeasuringtotalDnmt3Aamountfromtissuesorcells.Inanassaywiththiskit,theuniqueDnmtaffinitysubstrateisstablycoatedonthestripwell.ThesampleisaddedintothewellandDnmt3Acontainedinthesamplebindstothesubstrate.TheboundDnmt3Acanberecognizedwitha specificDnmt3AantibodyandcolorimetricallyquantifiedthroughanELISA-likereaction.TheamountofDnmt3Aisproportionaltotheintensityofcolordevelopment.

 
Fig.1.SchematicprocedureforusingtheEpiQuikDnmt3AAssayKit.

Fig.2. NuclearextractswerepreparedfromMCF-7cellsusingtheEpiQuikNuclearExtractionKitandtotalamountofDnmtwasmeasured.