Epigentek/EpiQuik Dnmt1 Assay Kit/P-3011-3/96 assays-免疫在线蚂蚁淘旗下平台

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商品详细Epigentek/EpiQuik Dnmt1 Assay Kit/P-3011-3/96 assays

Epigentek/EpiQuik Dnmt1 Assay Kit/P-3011-3/96 assays

商品编号： P-3011-3

品牌： Epigentek Group Inc

市场价： ¥11580.00

美元价： 6948.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

InputType:	NuclearExtracts,PurifiedEnzyme
ResearchArea:	DNAMethylation
TargetApplication:	AmountQuantitation
VesselFormat:	96-WellPlate
100%Guarantee:	6months

ProductOverview

TheEpiQuik™Dnmt1AssayKitisaconvenientpackageoftoolsthatallowstheexperimentertomeasuretheamountofDnmt1attremendouslyfastspeedsandconsistency;superiorandsaferthanallothercurrentmethods.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulDnmt1assayexperimentwithouttheneedforelectrophoresisandtransferprocess.TheEpiQuik™Dnmt1AssayKitissuitableformeasuringDnmtamountsquantitativelyfromfreshtissueandculturedcellsofhumanandmouse.Thekithasthefollowingadvantages:

Thefastestprocedure,whichcanbefinishedwithin3hours.
InnovativecolorimetricassaytoquantitativelymeasureDnmt1amountwithouttheneedforelectrophoresis.
StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™Dnmt1AssayKitisdesignedformeasuringtotalDnmt1amountfromtissuesorcells.Inanassaywiththiskit,theuniqueDnmtaffinitysubstrateisstablycoatedonthestripwell.ThesampleisaddedintothewellandDnmt1containedinthesamplebindstothesubstrate.TheboundDnmt1canberecognizedwithaspecificDnmt1antibodyandcolorimetricallyquantifiedthroughanELISA-likereaction.TheamountofDnmt1isproportionaltotheintensityofcolordevelopment.

Fig.1.SchematicprocedureforusingtheEpiQuikDnmt1AssayKit.

Fig.2. NuclearextractswerepreparedfromMCF-7cellsusingtheEpiQuikNuclearExtractionKitandtotalamountofDnmtwasmeasured.

ProductComponents

D1(10XWashBuffer)
D2(AssayBuffer)
D3(Dnmt1Standard,20µg/ml)*
D4(AffinityAntibody,500µg/ml)*
D5(DetectionAntibody,200µg/ml)*
D6(DevelopingSolution)
D7(StopSolution)
BB(BlockingBuffer)
8-WellAssayStrips(withFrame)
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialafterthawingpriortoopeningthecap.

品牌介绍

Epigentek快速，有效地制备DNA，蛋白质和染色质对于进行下游分析至关重要。我们的样品制备套件套件几乎适用于所有工作流程，无论是为ChIP-Seq或ChIP-PCR应用分离染色质，还是从各种起始原料制备亚硫酸氢盐-Seq或MS-PCR的DNA。我们的样品制备套件使您可以直接从提取到反应，为您的实验工作流程提供无数种可能性和灵活性。我们的DNA，蛋白质和染色质制备试剂盒是无数研究机会和下游应用的起点。

公司介绍

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.epigentek.com