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Epigentek/EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)/P-3009-48/48 assays
| InputType: | NuclearExtracts,PurifiedEnzyme |
| ResearchArea: | DNAMethylation |
| TargetApplication: | ActivityMeasurement |
| VesselFormat: | 96-WellPlate |
| 100%Guarantee: | 6months |
The EpiQuik™DNMT(DNAMethyltransferase)Activity/InhibitionAssayUltraKit isacompletesetofoptimizedbuffersandreagentsthatallowstheexperimentertocolorimetricallymeasure DNAmethyltransferase activityorinhibitionatextremelyfastspeedsona96-stripwellmicroplate.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulDNMTactivity/inhibitionexperimentwithouttheneedforrADIoactivityoranyspecialequipment. TheEpiQuikDNMTActivity/InhibitionAssayUltraKitisafurtherrefinementofitspopular predecessorkit byenhancingsamplesignalsandsignificantlyminimizingbackgroundsignals,inadditiontobeingfivetimesmoresensitive.Thekithasthefollowingadvantagesandfeatures:
- Colorimetricassaywitheasy-to-followstepsforconvenienceandspeed.Theentireprocedurecanbecompletedwithin3hoursand45minutes.
- Safeandinnovativecolorimetricassaywithoutradioactivity,extraction,andchromatography.
- Theultra-sensitivedetectionlimitcanbeaslowas0.5µgofnuclearextractor0.5ngofpurifiedenzymes,whichisfivetimesbetterthanthepredecessorkit.
- Optimizedantibody&enhancersolutionsallowhighspecificityto5-mCwithoutcross-reactivitytounmethylatedcytosine.
- Strip-wellmicroplateformatallowsforeitherloworhighthroughputanalysis.
The EpiQuikNuclearExtractionKit hasalsobeenoptimizedforusewiththiskit.
AboutDNAMethyltransferases
Theadditionofmethylgroupsiscarriedoutbyafamilyofenzymes,DNAmethyltransferases(DNMTsorDNAMTases).ChromatinstructureinthevicinityofgenepromotersalsoaffectsDNAmethylationandtranscriptionalactivity.DNMT1,DNMT3A,andDNMT3BarerequiredfortheestablishmentandmaintenanceofDNAmethylationpatterns.Twoadditionalenzymes,DNMT2/TRDMT1andDNMT3L,mayalsohavemorespecializedbutrelatedfunctions.DNMT1appearstoberesponsIBLeformaintenanceofestablishedpatternsofDNAmethylation,whileDNMT3Aand3Bseemtomediateestablishmentofnewordenovo,DNAmethylationpatterns.DNMT2/TRDMT1wasshowntomethylatetRNAAspatC38andDNMT3LisfoundtobeacatalyticallyinactiveregulatoryfactorofDNAmethyltransferases,whichisessentialforthefunctionofDNMT3AandDNMT3B.DiseasedcellssuchascancercellsmaybedifferentinthatDNMT1aloneisnotresponsibleformaintainingabnormalgenehypermethylationandbothDNMT1andDNMT3Bmaycooperatewiththisfunction.
MU1(10XWashBuffer)
MU2(DNMTAssayBuffer)
MU3(Adomet,50X)*
MU4(DNMTEnzymeControl,50 µg/ml)*
MU5(CaptureAntibody,1000 µg/ml)*
MU6(DetectionAntibody,400µg/ml)*
MU7(EnhancerSolution)*
MU8(DeveloperSolution)
MU9(StopSolution)
8-WellAssayStrips(WithFrame)
AdhesiveCoveringFilm
UserGuide
*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialafterthawingpriortoopeningthecap.
