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Epigentek/EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit/P-3001-1/48 assays
| InputType: | DNA |
| ResearchArea: | DNAMethylation |
| TargetApplication: | ActivityMeasurement |
| VesselFormat: | 96-WellPlate |
| 100%Guarantee: | 6months |
Werecommendusinganewerversionofthisproduct: EpiQuikDNMTActivity/InhibitionAssayUltraKit(Colorimetric)
The EpiQuik™DNAMethyltransferaseActivity/InhibitionAssayKit isacompletesetofoptimizedbuffersandreagentsthatallowstheexperimentertomeasureDNAmethyltransferaseactivityorinhibitionatextremelyfastspeeds.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulDNMTactivity/inhibitionexperimentwithouttheneedforrADIoactivityoranyspecialequipment.TheEpiQuik™DNAMethyltransferaseActivity/InhibitionAssayKitissuitableformeasuringDNMTactivity/inhibitionfromabroadrangeofspeciesincludingmammaliancells/tissues,plants,andbacteria.Thekithasthefollowingadvantages:
- Veryrapidprocedure,whichcanbecompletedwithin3hours.
- Safeandinnovativecolorimetricassaywithoutradioactivity,extraction,andchromatography.
- StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
- Extremelysimple,reliable,andconsistentassayconditions.
Principle&Procedure
TheEpiQuik™DNAMethyltransferaseActivity/InhibitionAssayKitisdesignedformeasuringtotalDNMTactivity(denovo,maintenance).Inanassaywiththiskit,theuniquecytosine-richDNAsubstrateisstablycoatedonthestripwells.ThesewellsarespecificallytreatedtohaveahighDNAabsorptionABIlity.DNMTenzymestransferamethylgrouptocytosinefromAdomettomethylateDNAsubstrate.ThemethylatedDNAcanberecognizedwithananti-5-methylcytosineantibody.TheratiooramountofmethylatedDNA,whichisproportionaltoenzymeactivity,canthenbecolorimetricallyquantifiedthroughanELISA-likereaction.
M1(10XWashBuffer)
M2(DNMTAssayBuffer)
M3(Adomet,8mM)*
M4(DNMTPositiveControl)*
M5(CaptureAntibody)*
M6(DetectionAntibody,200µg/ml)*
M7(DevelopingSolution)
M8(StopSolution)
8-WellSubstrate-CoatedStrip(withFrame)
UserGuide
*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialafterthawingpriortoopeningthecap.
