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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > Epigentek/EpiQuik MBD2 Binding Activity/Inhibition Assay Kit/P-3021-96/96 assays
商品详细Epigentek/EpiQuik MBD2 Binding Activity/Inhibition Assay Kit/P-3021-96/96 assays
Epigentek/EpiQuik MBD2 Binding Activity/Inhibition Assay Kit/P-3021-96/96 assays
Epigentek/EpiQuik MBD2 Binding Activity/Inhibition Assay Kit/P-3021-96/96 assays
商品编号: P-3021-96
市场价: ¥11580.00
美元价: 6948.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
InputType:DNA
ResearchArea:DNAMethylation
TargetApplication:ActivityMeasurement
VesselFormat:96-WellPlate
100%Guarantee:6months
ProductOverview

TheEpiQuik™MBD2BindingActivity/InhibitionAssayKitisaconvenientsetoftoolsthatallowstheexperimentertomeasurebindingactivityofMBD2tomethylatedDNA.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulactivity/inhibitionexperimentwithouttheneedforrADIoactivityoranyspecialequipment.TheEpiQuik™MBD2BindingActivityAssayKitissuitableformeasuringMBD2bindingactivityinhumancells/tissues.Thekithasthefollowingadvantages:

  • Thefastestprocedureavailable,whichcanbefinishedwithin3hours.
  • InnovativecolorimetricassaytoquantitativelymeasureMBD2binding.
  • StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
  • Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™MBD2BindingActivityAssayKitisdesignedformeasuringinvitroorintracellularMBD2bindingactivity.Inanassaywiththiskit,activatedMBD2proteincontainedinnuclearextractbindstomethylatedDNAandformstheMBD2/methylatedDNAcomplex.Thecomplexisthencapturedtoaspecifically-treatedstripwellplate.MBD2isrecognizedwithaspecificantibodyandmeasuredcolorimetrically.

 
Fig.1.SchematicprocedureforusingtheEpiQuikMBD2BindingActivity/InhibitionAssayKit.

ProductComponents

MB1(10XWashBuffer)
MB2(AssayBuffer)
MB3(MethylatedDNA,25µg/ml)*
MB4(AffinityAntibody,50µg/ml)*
MB5(DetectionAntibody,200µg/ml)*
MB6(DevelopingSolution)
MB7(StopSolution)
8-WellAssayStrips(withframe)
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialafterthawingpriortoopeningthecap.

品牌介绍
Epigentek快速,有效地制备DNA,蛋白质和染色质对于进行下游分析至关重要。我们的样品制备套件套件几乎适用于所有工作流程,无论是为ChIP-Seq或ChIP-PCR应用分离染色质,还是从各种起始原料制备亚硫酸氢盐-Seq或MS-PCR的DNA。我们的样品制备套件使您可以直接从提取到反应,为您的实验工作流程提供无数种可能性和灵活性。我们的DNA,蛋白质和染色质制备试剂盒是无数研究机会和下游应用的起点。