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Epigentek/BisulFlash DNA Bisulfite Conversion Easy Kit/P-1054-050/50 reactions
| InputType: | DNA |
| ResearchArea: | DNAMethylation |
| TargetApplication: | SampleModification |
| VesselFormat: | Columns/Tubes |
| 100%Guarantee: | 6months |
The BisulFlash™DNABisulfiteConversionEasyKit usesauniqueconversionsolutionandcolumn-basedclean-uptogeneratebisulfite-convertedDNAinafast,reliable,andconvenientformat.Eachkitcontainssufficientcomponentsfor50totalreactions.Highyield,convertedDNAcanbeobtainedandusedforvariousdownstreamapplicationsincludingPCR,array,bisulfitesequencing,aswellasnextgenerationsequencing.Thekithasthefollowingadvantages:
- FastProcedure– Theentireprocesscanbecompletedwithin1hour.
- Streamlined- ConcurrentlyprocessestheDNAdenaturationandCtoTconversionstepswithouttheneedforaseparateDNAdenaturationstep.
- Convenient -Thebisulfiteconversionreagentisinastable,pre-mixedliquidsolutionformat,eliminatingtheneedforpowder-basedpreparation.
- CompleteConversion- Completelyconvertsunmethylatedcytosineintouracil(>99.9%)withnegligIBLeinappropriateorerroneousconversionofmethylcytosinetothymine(<0.1%).
- Robust- Simple,reliable,andconsistentreactionconditionswithaneasy-to-followprotocolandhighyield.
BackgroundInformation
DNAmethylation isessentialinregulatinggeneexpressioninnearlyallBIOLOGicalprocessesincludingdevelopment,growth,anddifferentiation.Gene/region-specificorgenome-wideanalysisofDNAmethylationor5-methylcytosine(5-mC)couldprovidevaluableinformationfordiscoveringepigeneticMarkersusedfordiseasediagnosis,andpotentialtargetsusedfortherapeutics.BytreatingDNAwithbisulfite,cytosineresiduesaredeaminatedtouracilwhileleaving5-methylcytosineintact,sothatmethylatedcytosinecanbeproperlyidentifiedviaPCR,array,bisulfitesequencing,ornextgenerationsequencing.
Principle&Procedure
Thiskitcontainsallreagentsnecessaryforafastbisulfiteconversioninahighthroughputformat.Withtheuniqueconversionmixsolution,DNAdenaturationstatusissustainedthroughouttheentirebisulfiteconversionprocess,therebyenablingallunmethylatedcytosinestobesuccessfullyconvertedtouracil.Desulfonationandclean-upoftheconvertedDNAisperformedusingDNApurificationcolumns.Highyield,convertedDNAcanbeobtainedandusedforvariousdownstreamapplicationsincludingPCR,array,andnextgenerationsequencing.
StartingMaterials
Startingmaterialsmayincludevarioustissueorcellsamplessuchas:culturedcellsfromaflaskormicroplate,microdissectionsample,paraffin-embeddedtissue,plasma/serumsample,andbodyfluidsample,etc
BisulFlashConversionMix
DNABindingSolution
DesulfonationBuffer
ElutionBuffer
SpinColumn*
CollectionTube
UserGuide
*Alwayscapspincolumnsbeforeplacingtheminthemicrocentrifuge.
