Epigentek/BisulFlash DNA修饰试剂盒/P-1026-050/50反应-免疫在线蚂蚁淘旗下平台

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商品详细Epigentek/BisulFlash DNA修饰试剂盒/P-1026-050/50反应

Epigentek/BisulFlash DNA修饰试剂盒/P-1026-050/50反应

商品编号： P-1026-050

品牌： Epigentek Group Inc

市场价： ¥2580.00

美元价： 1548.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

InputType:	DNA
ResearchArea:	DNAMethylation
TargetApplication:	SampleModification
VesselFormat:	Columns/Tubes
100%Guarantee:	6months

ProductOverview

TheBisulFlash™DNAModificationKitisacompletesetofoptimizedbuffersandreagentstoperformDNAmodificationusinganexpeditedDNAbisulfiteconversiontechnologydevelopedbyEpigentek,gearedspecificallyformethylation-specificPCR(MSP)aswellaspost-bisulfitebasedNGSapplications.ThroughaproprietarycompositionwhichallowsDNAdenaturationandbisulfiteconversiontobeprocessedatthesametime,thecompleteprocedureisreducedtoonly30minutes.FurThermore,itpreventsmorethan90%ofDNAloss,completelyconvertingunmethylatedcytosineintouracil.

PerfectingBisulfiteConversion
TrADItionalmethodsinvolveaseparatedenaturationstepfollowedbyasubsequentsodiumbisulfiteDNAconversionstep--butwiththeBisulFlash™method,DNAdenaturationstatusisconcurrentlysustainedthroughouttheentireDNAbisulfiteconversionprocess.ThisbreakthroughapproachenablestheDNAconversionprocesstobesignificantlyfasterwithhigherconversionefficiencyandaccuracy.WecontinuetoinnovatewiththedevelopmentofthenewBisulFlash™kitbyidentifyingfourcriticalcomponentsofbisulfiteconversion:

Speed:Reducetheentireproceduretoasshortas30minuteswithoutanyreagentsetuptime.
Efficiency:Completelyconvertunmethylatedcytosineintouracil--modifiedDNA>99.9%.
DNAProtection:ProtectagainstDNAdegradationofwhichmorethan90%ofDNAlosscanbeprevented,allowingforgreaterrecovery.
Sensitivity:StartwiththelowestamountofinputDNAformodification--only0.2ngorjust50cells.

Theconvenientready-to-use,DNAconversionmixsolutionandsingletemperatureincubationalongwiththefeaturesmentionedaboveallowfortrueperfectioninbisulfiteconversion.TheBisulFlash™DNAModificationKitissuitableforMS-PCR/MSPandrealtimeMSP.

BisulfiteSequencingOption
The BisulFlash™kitincludesaspecialstepmadespecificallyforbettersequencingresultsandis100%compatIBLewithallIllumina®platforms.ForidealNGSapplications,werecommendcombiningthistechniquewithourEpiNextPost-BisulfiteLibraryPreparationKit.

Fig.1.HighaccuracyofDNAconversionisachievedbythe BisulFlash™DNAModificationKit.50ngofgenomicDNAmethylatedinallCpGsitesbyDNAmethylaseswastreatedwiththe BisulFlash™DNAModificationKit.ConvertedDNAwasamplifiedbyrealtimeqPCRusingprimersformultiplepromoterscontainingnumerousCpGsitesandthendirectlysequenced.

ComparativeOverviewofCommercialKits
Datawasobtainedthroughactualuseofthekit,customerfeedback,orinformationprovidedbythesupplier"sdatasheetorwebsite.

	BisulFlash	Supplier1	Supplier2	Supplier3
ProcessingTime	30min	>3hours	>6hours	12-16hours
CorrectConversion	99.9%	99.5%	99.4%	98%
ErrorConversion	<0.1%	>0.3%	N/A	>1.5%
Correct/ErrorConversionRatio	≅1000	≅330	N/A	≅65
DNADegradation	Verylow	Medium	Low	High
MinimumStartingDNA	0.2ng	0.5ng	1ng	1ng
Convenience	Veryhigh	High	Medium	Low

ProductComponents

BF1(ConversionMixSolution)
BF2(CaptureSolution)
BF3(DesulphonationSolution)
BF4(ElutionSolution)
BF5(ConversionEnhancer)
BF6(DenaturationEnhancer)
F-SpinColumns
F-CollectionTubes
UserGuide

品牌介绍

Epigentek快速，有效地制备DNA，蛋白质和染色质对于进行下游分析至关重要。我们的样品制备套件套件几乎适用于所有工作流程，无论是为ChIP-Seq或ChIP-PCR应用分离染色质，还是从各种起始原料制备亚硫酸氢盐-Seq或MS-PCR的DNA。我们的样品制备套件使您可以直接从提取到反应，为您的实验工作流程提供无数种可能性和灵活性。我们的DNA，蛋白质和染色质制备试剂盒是无数研究机会和下游应用的起点。

公司介绍

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.epigentek.com