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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > Epigentek/DNA Methylation/Hydroxymethylation Standard Set/P-1025-1/2 µg/standard x 3
商品详细Epigentek/DNA Methylation/Hydroxymethylation Standard Set/P-1025-1/2 µg/standard x 3
Epigentek/DNA Methylation/Hydroxymethylation Standard Set/P-1025-1/2 µg/standard x 3
Epigentek/DNA Methylation/Hydroxymethylation Standard Set/P-1025-1/2 µg/standard x 3
商品编号: P-1025-1
市场价: ¥7180.00
美元价: 4308.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
InputType:DNA
ResearchArea:DNAMethylation
TargetApplication:AssayValidation
VesselFormat:Columns/Tubes
100%Guarantee:6months
ProductOverview

TheDNAMethylation/HydroxymethylationStandardSetisdesignedtobeusedaspositivecontrolsinDNAmethylationexperimentsandvariousapplicationsincludingMS/hMS-PCRandquantificationofcytosinemodificationsinanyspecies.Itcanalsobeusedassubstratesforstudiesinvolving5-methylcytosine(5mC)and5-hydroxymethylcytosine(5hmC) interactingproteins.

  • IncludesthreeDNAstandards:unmodifiedDNAcontainingonlycytosinesasanegativecontrol,methylatedDNAcontainingonly5-methylcytosines(5-mC),andhydroxymethylatedDNAcontainingonly5-hydroxymethylcytosines(5-hmC).
  • Eachstandardisa200basepairfragmentthatwasgeneratedbyPCRusingtheMethylamp™MS-qPCRFastKit(Cat.#P-1028)andcontains57cytosineresiduesontheforwardstrand,ofwhich19areatCpGsites.
  • EachDNAstandardisconfirmedbyelectrophoresisonanagarosegel.
  • TheincludedPCRprimerscontainbothforwardandreversestrandprimerstoamplifya127basepairPCRproductoftheDNAstandard.ThesePCRprimerscanbeusedforeitherendpointorrealtimePCR.


Fig.1. EachDNAstandard(500ng)wasrunona2.5%agarosegel.AllthreeDNAstandardsare200bpandeitherunmodified(cytosine,Lane1),methylated(5-mC,Lane2)orhydroxymethylated(5-hmC,Lane3).

ProductComponents

3x2µgperstandard(50ng/ul)

Unmodifiedcontrolprimer(20 µM)
Forward:10 µl
Reverse:10ul

Modifiedcontrolprimer(20 µM)
Forward:10 µl
Reverse:10 µl

品牌介绍
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