TheMethylamp™96DNAModificationKitisacompletesetofessentialcomponentswhichenablestheexperimentertomodifyDNAefficiently.Theentireprocedurecanbecompletedwithin2hoursand30minutesandproducesfarsuperiorresultsthananycompetitorkits.TheMethylamp™96DNAModificationKitissuitableforMS-PCR,realtimeMS-PCR,methylationsequencing,andpyrosequencing,aswellasmethylationmicroarray.Thekithasthefollowingadvantages:

• Thefastestprocedureavailable,whichcanbefinishedwithin2hoursand30min.
• Highthroughputisachievablealongwitha96-wellfilterformat.
• Completelyconvertsunmethylatedcytosineintouracil:modifiedDNA>99.98%.
• ThelowestdegradationofDNAinthemodificationprocess:morethan90%ofDNAlosscanbeprevented.
• ThelowestrequirementofstartingDNAformodification:only1ng.
• Simple,reliable,andconsistentmodificationconditions.

Principle&Procedure
TheMethylamp™96DNAModificationKitcontainsallreagentsrequiredforbisulfiteconversiononaDNAsample.DNAisdenaturedbyheating,whichallowsDNAdenaturationandbisulfitemodificationtobecarriedoutsimultaneously.Inthemodificationprocess,bisulfitereagentreactsspecificallywithsingle-strandedDNA,therebydeaminatingcytosineandcreatingauracilresidue.TheuniqueDNAprotectionreagentscontainedinthemodificationbuffercanpreventthechemicalandThermophilicdegradationofDNAinthebisulfitetreatment.Thenon-toxicmodifiedDNAcapturebufferenablesDNAtotightlybindtothecolumnfilter,thusDNAcleaningcanbecarriedoutonthecolumntoeffectivelyremoveresidualsodiumbisulfiteandsalts.ModifiedDNAthencanbeelutedandstablystoredat-20°Cforupto2months.

 
Fig.1.SchematicprocedureforusingtheMethylamp96DNAModificationKit