The EpiNext™Hi-FiCDNASynthesisKit isacompletesetofoptimizedreagents tosynthesizeaDNAcopyspecificallyusingbisulfite-convertedRNAandenrichedRNAfragments. 

• OptimizedtosynthesizeDNAcopiesfrombisulfite-convertedRNA
• AlsosuitableforcDNAsynthesisfromtotalRNA
• SynthesizedDNAisfullycompatIBLewithPCRandcDNAlibraryconstruction
• IncludedRTenzymehaslowinstrinsicRNaseactivitytodeliverextremelyhighreversetranscriptionaccuracyandpromotesfull-lengthcDNAsynthesis

Description
EffectiveandefficientpreparationofcDNAfromRNAcanhelpensurereliabledatainvariousdownstreamapplicationsincludingreal-timeqPCRandRNA/cDNA-sequencing.TheEpiNext™ Hi-FicDNASynthesisKithasbeenoptimizedandvalidatedforcDNAsynthesisfromvariousRNAsamples,particularlyfrombisulfite-convertedRNAandenrichedRNAfragments.ThekitcontainsallnecessarycomponentsincludingahighlysensitiveandThermostablerecombinantreversetranscriptasewhichprovidesenhancedcDNAsynthesisefficiency,highfidelity,andawiderangeofactivityforvaryingamountsofRNAtemplates(0.1ngto2 µg).Due toitshighthermalstABIlity,lowRNaseactivityandresistancetoinhibitionbyrRNAandtRNA,therecombinantRTincludedinthekitcanbeusedforsynthesisoflongcDNAproductsattemperaturesfrom42°C-60°CusingtotalRNA,andGC-richtemplatessuchasbisulfiteconvertedRNA.ArecombinantRNaseinhibitorisalsoincludedinthekittopreventthedegradationoftargetRNAduetoribonucleasecontamination.

StartingMaterials
RNAisolatedfromvarioustissueorcellsamplescanbeusedasstartingmaterial.TheamountofRNAforeachreactioncanbe0.1ngto2µg.Foranoptimalreaction,theinputRNAamountshouldbe200ngto500ng.

 Fig.1. TheEpiNextHiFicDNASynthesisKitmechanismusesbothA)oligo-dTandB)randomhexamers.