TheEpiNext™ DNASizeSelectionKitisacompletesetofoptimizedreagentsforquickremovalofDNAfragmentsof<150bpsforlibrarypreparationinnextgenerationsequencingapplications.ThekitutilizesmagneticbeadtechnologyandissuitableforIllumina, LifeTechnologies(SOLiD), IonTorrent,andRoche/454platforms.ItcanalsobeusedforremovingundesiredlargerDNAfragmentsbyoptimizingthebeadtoDNAvolumeratioaccordingly.Theindicatednumberofreactionscanbeperformedforastandard50µlsolutioninputDNAsample.Thekithasthefollowingadvantages:

• Optimizedfragmentselectionchemistries:CompleteseparationofDNAfragmentsaccordingtosize.SuitableforIllumina, IonTorrent,SOLiDand Roche/454platforms.
• Fastandstraightforward: Sizeselectionprocedurecanbefinishedwithin30min.Nogels,columnsorcentrifugationareneeded.
• Efficientremovalofprimer-dimers:Noneedforfurtherclean-upsteps.
• HighrecoveryoftargetedDNAfragments:Higherthan85%recoveryofinputDNAfragments. 
• Manualandautomationfriendly:Scalableforuseinsingletubeor96-wellplateformat.

BackgroundInformation
DNAsizeselectionisnecessaryafterDNAshearingaspartofthelibraryconstructionprocessfornextgenerationsequencing,regardlessoftheplatformused.ObtaininghighrecoveryofselectedDNAfragmentsiscriticalforthereductionofsequencingbias. 

Principle&Procedure
ThiskitincludesallreagentsrequiredateachstepoftheworkflowforDNAsizeselection.ThesizeofDNAfragmentsboundtotheincludedMQBindingBeadsisbasedontheratioofMQbeadstotheDNAsamplesolution.OptimizationofMQbeadratiotoinputDNA,allowsfortheremovaloflargerorsmallerDNAfragmentsandrecoveryofdesiredtargetsizeDNAfragments.

StartingMaterials
DNAfragmentsofvariouslengths.Inputamountcanbefrom0.1ngto1µg.  

Fig.1. WorkflowoftheEpiNext™ DNASizeSelectionKit.

Fig.2. SizeselectionofDNAfragments:HumanPlacentaDNAwasshearedto100-700bpsinlengthwithpeaksizeofabout300bps(A).Atargetpeaksizeof500bps(B)wasselectedusingtheEpiNext™ DNASizeSelectionKit.