InputType: | DNA |
ResearchArea: | DNAMethylation,NextGenSequencing |
TargetApplication: | LibraryConstruction |
VesselFormat: | 96-WellPlate |
100%Guarantee: | 6months |
TheEpiNext™ DNASizeSelectionKitisacompletesetofoptimizedreagentsforquickremovalofDNAfragmentsof<150bpsforlibrarypreparationinnextgenerationsequencingapplications.ThekitutilizesmagneticbeadtechnologyandissuitableforIllumina, LifeTechnologies(SOLiD), IonTorrent,andRoche/454platforms.ItcanalsobeusedforremovingundesiredlargerDNAfragmentsbyoptimizingthebeadtoDNAvolumeratioaccordingly.Theindicatednumberofreactionscanbeperformedforastandard50µlsolutioninputDNAsample.Thekithasthefollowingadvantages:
BackgroundInformation Principle&Procedure StartingMaterials |
Fig.2. SizeselectionofDNAfragments:HumanPlacentaDNAwasshearedto100-700bpsinlengthwithpeaksizeofabout300bps(A).Atargetpeaksizeof500bps(B)wasselectedusingtheEpiNext™ DNASizeSelectionKit. |
MQBindingBeads*
ElutionSolution*
96-wellplate
UserGuide
*Spinthesolutiondowntothebottompriortouse.