TheChromaFlash™PlantChromatinExtractionKitisacompletesetofoptimizedbuffersandreagentsforisolatingchromatinorDNA-proteincomplexesfromplantsinasimpleandrapidformat.Chromatinpreparedbythiskitcanbeusedinavarietyofchromatinimmunoprecipitationmethods.ItisalsotherecommendedmethodforobtainingchromatinrequiredbyEpigentek’sone-hourChIPmethodusingtheChromaFlash™One-StepChIPKitortheChromaFlash™One-StepMagneticChIPKit.Theisolatedchromatincanalsobeusedinotherchromatin-relatedapplicationssuchasinvitroprotein-DNAbindingassays,ornuclearenzymeassays.

• Extremelyfastprocedure:theentireprocedurefromplantsamplestoready-to-usechromatinislessthan2hours.
• ConvenientandflexIBLe:thekitissuitableforpreparingbothnativechromatinandcross-linkedchromatinfrommonolayerorsUSPensioncells,orfromtissues. 
• Unshearedchromatinmakesitcustomizableforvariousanalysisworkflowsthatrequireeitherintactorfragmentedchromatin,includingChIP,invitroprotein-DNAinteractionanalysis,nuclearenzymeassays,etc.

BackgroundInformation
Chromatinimmunoprecipitation(ChIP)offersanadvantageoustoolforstudyingprotein-DNAinteraction.WithChIP,theexperimentercandetermineifaspecificproteinbindstothespecificsequencesofageneinlivingcellsbycombiningitwithPCR(ChIP-PCR),microarray(ChIP-chip),orsequencing(ChIP-Seq)techniques.Forexample,themeasurementoftheamountofmethylatedhistoneH3atlysine9(meH3-K9)associatedwithaspecificgenepromoterregionundervariousconditionscanbeachievedthroughaChIP-PCRassay,whilerecruitmentofmeH3-K9tothepromotersonagenome-widescalecanbedetectedbyChIP-chip.Inparticular,aChIPmethodwithspecificantibodiesdirectlyagainstvarioustranscriptionalfactorsiswidelyindemand.

Fig.1. SchematicprocedureoftheChromaFlash™ PlantChromatinExtractionKit.

Fig.2.ChIPanalysisoftri-methylH3-K27enrichedinAGAMOUSgenewithchromatinextractpreparedfrom2-week-oldicu2-1/icu2-1seedlingsusingtheChromaFlash™ PlantChromatinExtractionKit.